PXD032958

PXD032958 is an original dataset announced via ProteomeXchange.

Title	Functional genomics of glioblastoma: from epigenetics to proteomic investigation of tumor-initiating cell secretome
Description	The main objectives of the project are as follows: 1) Deciphering the TIC specific secretome signature. The secretome collected from four different TICs and their corresponding differentiated cell cultures will be subjected to label-free quantitative proteomic analysis to identify a TIC specific secretome signature. 2) Validation and functional characterization of selected molecules from TIC specific secretome signature. Selected molecules based on the level of regulation and literature information will be validated at the level of transcript and protein. The importance of a set of validated proteins will be studied both in vitro using neurosphere cultures and angiogenesis assays, and in vivo using an intracranial glioma model in nude mice 3) Characterizing of GBM specific serum proteome signature. Using label-free quantitative proteomics, we will compare the serum proteome of tumor bearing mice (xenotransplantated with TICs), early after tumor establisment and after the tumor has reached its maximum size.We will also explore by targeted quantitative proteomics whether the level of “specific” TIC-secreted proteins is increased in the serum of TIC initiated GBM bearing mice and verify, using the same strategy, proteins found to be differentially expressed in serum in both mice groups. These studies will reveal whether TIC-secreted proteins (and/or other GBM-derived proteins) can be retrieved in serum and thus be considered as candidate biomarkers of GBM.
HostingRepository	PRIDE
AnnounceDate	2022-06-07
AnnouncementXML	Submission_2022-06-07_00:17:15.372.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Functional Proteomics Platform FPP
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-03 07:23:03	ID requested
⏵ 1	2022-06-07 00:17:19	announced

Sengupta S, Mondal M, Prasasvi KR, Mukherjee A, Magod P, Urbach S, Friedmann-Morvinski D, Marin P, Somasundaram K, Differentiated glioma cell-derived fibromodulin activates integrin-dependent Notch signaling in endothelial cells to promote tumor angiogenesis and growth. Elife, 11():(2022) [pubmed]

submitter keyword: Proteomics, tumor-initiating cells, cancer stem cells, secretome

Prof. Kumaravel Somasundaram
contact affiliation	Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India
contact email	skumar1@iisc.ac.in
lab head
Functional Proteomics Platform FPP
contact affiliation	FPP CNRS
contact email	contact@fpp.cnrs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD032958

PRIDE project URI

• PRIDE
  1. PXD032958
     1. Label: PRIDE project
     2. Name: Functional genomics of glioblastoma: from epigenetics to proteomic investigation of tumor-initiating cell secretome