PXD032957
PXD032957 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Investigating peptide-Coenzyme A-conjugates as chemical probes for proteomic profiling of N-terminal and lysine acetyltransferases |
| Description | Acetyl groups are transferred from Acetyl-Coenzyme A (Ac-CoA) to protein N-termini and lysine side chains by N-terminal acetyltransferases (NATs) and lysine acetyltransferases (KATs), respectively. Building on lysine-CoA conjugates as KAT probes, we have synthesized peptide probes with CoA conjugated to N-terminal alanine (α-Ala-CoA), proline (α-Pro-CoA) or tri-glutamic acid (α-3Glu-CoA) units for interactome profiling of NAT complexes. These probes recruited catalytic and auxiliary subunits of the major NAT complexes from cell lysates specifically. The α-Ala-CoA probe was more specific in recruiting NAT catalytic and auxiliary subunits when compared to a lysine CoA-conjugate which bound only a subset of endogenous KATs. Interactome profiling with the α-Pro-CoA probe showed reduced NAT recruitment in favor of metabolic CoA binding proteins, while α-3Glu-CoA steered the interactome towards NAA80 and NatB. These findings agreed with the inherent substrate specificities of the target proteins and showed that N-terminal CoA-conjugated peptides are versatile probes for NAT complex profiling in lysates of physiological and pathological backgrounds. |
| HostingRepository | jPOST |
| AnnounceDate | 2022-05-02 |
| AnnouncementXML | Submission_2023-02-06_06:14:42.665.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jürgen Eirich |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-04-03 02:58:17 | ID requested | |
| 1 | 2022-05-02 05:30:13 | announced | |
| 2 | 2022-09-18 02:21:30 | announced | 2022-09-18: Updated FTP location. |
| ⏵ 3 | 2023-02-06 06:14:43 | announced | 2023-02-06: Updated PubMed. |
Publication List
| Sindlinger J, Sch, ö, n S, Eirich J, Kirchg, ä, ß, ner S, Finkemeier I, Schwarzer D, Investigating Peptide-Coenzyme A Conjugates as Chemical Probes for Proteomic Profiling of N-Terminal and Lysine Acetyltransferases. Chembiochem, 23(17):e202200255(2022) [pubmed] |
Keyword List
| submitter keyword: Acetylation, NAT, KAT, CoA |
Contact List
| Iris Finkemeier | |
|---|---|
| lab head | |
| Jürgen Eirich | |
| contact affiliation | University of Münster - WWU - Institute for Biology and Biotechnology of Plants |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001556/ |
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