PXD032955

PXD032955 is an original dataset announced via ProteomeXchange.

Title	Identification of gamma-carboxyglutamic acid residues in mouse ERGP by LC-MS/MS.
Description	Vitamin K (VK) is a micronutrient required for the gamma-carboxylation of secreted proteins. Clinical studies have recently implicated VK in the pathophysiology of diabetes. Our data indicate that rodent beta-cells lacking gamma-carboxylation fail to adapt their insulin secretion in response to glucose in the context of age-related insulin resistance or diet-induced beta-cell stress. In contrast, VK supplementation protects beta-cells from endoplasmic reticulum stress-induced apoptosis. Using anti-Gla immunoprecipitation, we identified ERGP as a putative gamma-carboxylated protein present in pancreatic beta-cells. We used LC-MS/MS to confirm the presence of gamma-carboxyglutamic residues in ERGP.
HostingRepository	PRIDE
AnnounceDate	2023-05-10
AnnouncementXML	Submission_2023-05-10_07:32:02.774.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD032955
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	MathieuFerron
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	carboxylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-02 15:02:54	ID requested
⏵ 1	2023-05-10 07:32:03	announced

10.6019/PXD032955;

submitter keyword: Junctate, gamma-carboxylation, Mouse,Vitamin K, LC-MS/MS

MathieuFerron
contact affiliation	Molecular Physiology Research Unit, Institut de Recherches Cliniques de Montréal, Montréal, Québec, Canada, H2W 1R7
contact email	mathieu.ferron@ircm.qc.ca
lab head
MathieuFerron
contact affiliation	IRCM
contact email	mathieu.ferron@ircm.qc.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032955
     1. Label: PRIDE project
     2. Name: Identification of gamma-carboxyglutamic acid residues in mouse ERGP by LC-MS/MS.