PXD032952

PXD032952 is an original dataset announced via ProteomeXchange.

Title	Proximity labelling of FLAG-TurboID- or Myc-UltraID-tagged full-length RNPS1 or RNPS1 deletion mutants followed by proteomics analysis
Description	RNPS1 is a component of the EJC-associated ASAP and PSAP complex. We used full-length RNPS1, RNPS1 with a C-terminal deletion and the RNPS1 RRM in a proximity labeling approach. We aimed to confirm interaction partners of RNPS1 previously identified by MS of RNPS1 IPs using the same RNPS1 deletion mutants (Project 2209 and 2236). By identifying RNPS1 interaction partners we want to uncover how RNPS1 is able to fulfill its splicing regulatory functions.
HostingRepository	PRIDE
AnnounceDate	2022-05-16
AnnouncementXML	Submission_2022-05-16_06:03:06.564.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Prerana Wagle
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-02 14:29:32	ID requested
⏵ 1	2022-05-16 06:03:06	announced

Dataset with its publication pending

submitter keyword: Exon junction complex, alternative splicing, RNPS1

Niels H. Gehring
contact affiliation	Institute for Genetics, University of Cologne, 50674 Cologne, Germany
contact email	ngehring@uni-koeln.de
lab head
Prerana Wagle
contact affiliation	CECAD Research Center
contact email	proteomics-facility@uni-koeln.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD032952

PRIDE project URI

• PRIDE
  1. PXD032952
     1. Label: PRIDE project
     2. Name: Proximity labelling of FLAG-TurboID- or Myc-UltraID-tagged full-length RNPS1 or RNPS1 deletion mutants followed by proteomics analysis