PXD032934

PXD032934 is an original dataset announced via ProteomeXchange.

Title	Multiple myeloma-derived extracellular vesicles modulate the bone marrow immune microenvironment
Description	One of the hallmarks of multiple myeloma (MM) is a permissive BM microenvironment. Increasing evidence suggest that cell-to-cell communication between myeloma and immune cells via tumor cell-derived extracellular vesicles (EV) plays a key role in the pathogenesis of MM. Hence, we aimed to explore BM immune alterations induced by MM-derived EV. For this, we inoculated immunocompetent BALB/cByJ mice with a myeloma cell line - MOPC315.BM -, inducing a MM phenotype. Upon tumor establishment, characterization of the BM microenvironment revealed the expression of both activation and suppressive markers by lymphocytes, such as Granzyme b and PD-1, respectively. In addition, conditioning of the animals with MOPC315.BM-derived EV, before transplantation of the MOPC315.BM tumor cells, did not anticipate the disease phenotype. However, it induced features of suppression in the BM milieu, such as an increase in PD-1 expression by CD4+ T cells. Overall, our findings reveal the involvement of MOPC315.BM-derived EV protein content as promoters of immune niche remodeling, strengthening the importance of assessing the mechanisms by which MM may impact the immune microenvironment.
HostingRepository	PRIDE
AnnounceDate	2022-07-05
AnnouncementXML	Submission_2022-07-05_08:37:23.090.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rune Matthiesen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-02 13:36:59	ID requested
⏵ 1	2022-07-05 08:37:23	announced

Lopes R, Caetano J, Ferreira B, Barahona F, Carneiro EA, Jo, ã, o C, The Immune Microenvironment in Multiple Myeloma: Friend or Foe? Cancers (Basel), 13(4):(2021) [pubmed]

submitter keyword: Mouse , extracellular vesicles, multiple myeloma, bone marrow, microenvironment

Rune Matthiesen
contact affiliation	NMS, CEDOC, NOVA University Lisbon, Portugal
contact email	runem2009@gmail.com
lab head
Rune Matthiesen
contact affiliation	Computational and Experimental Biology Group, CEDOC NOVA MEDICAL SCHOOL / FACULDADE DE CIÊNCIAS MÉDICAS UNIVERSIDADE NOVA DE LISBOA Rua Câmara Pestana, 6-6A | 1150-082 Lisboa Portugal
contact email	runem2009@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032934
     1. Label: PRIDE project
     2. Name: Multiple myeloma-derived extracellular vesicles modulate the bone marrow immune microenvironment