PXD032926

PXD032926 is an original dataset announced via ProteomeXchange.

Title	Species identification of early colonial bone artefacts excavated from Pyrmont, Australia, by mass spectrometric identification of collagen peptides
Description	Zooarchaeology by Mass Spectrometry (ZooMS) is a rapidly developing and increasingly utilised peptide mass fingerprinting (PMF) technique that analyses Collagen 1A1 and 1A2 marker peptides for the genus- or species-level identification of fragmentary bones in the archaeological record. Traditionally, this analysis is performed using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-ToF-MS) to identify characteristic m/z values of known marker peptides. Here we present data on the application of a modified ZooMS approach, using nanoflow liquid chromatography – tandem mass spectrometry proteomics, to the analysis of a collection of six early colonial Australian (early to mid-19th Century CE) bone artefacts excavated from a site in Pyrmont, Sydney, Australia in 2017. We were successfully able to identify characteristic marker peptides for bovine COL1A1 and COL1A2 in all six artefacts.
HostingRepository	PRIDE
AnnounceDate	2023-11-29
AnnouncementXML	Submission_2023-11-28_20:59:02.558.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dylan Multari
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	monohydroxylated proline; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-02 12:57:22	ID requested
⏵ 1	2023-11-28 20:59:02	announced

10.1016/J.JASREP.2022.103740;

submitter keyword: LC-MSMS,Collagen

Paul Andrew Haynes
contact affiliation	Macquarie University
contact email	paul.haynes@mq.edu.au
lab head
Dylan Multari
contact affiliation	Macquarie University
contact email	dylan.multari@hdr.mq.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032926
     1. Label: PRIDE project
     2. Name: Species identification of early colonial bone artefacts excavated from Pyrmont, Australia, by mass spectrometric identification of collagen peptides