PXD032921

PXD032921 is an original dataset announced via ProteomeXchange.

Title	Post-translational changes in Phialophora verrucosa via lysine lactylation during prolonged presence in a patient with a CARD9-related immune disorder
Description	Lysine lactylation (Kla) is a newly discovered histone post-translational modification (PTM), playing important roles in regulating transcription in macrophages. Increasing evidence demonstrates that lysine lactylation plays an important regulatory role in metabolic processes in both bacterial and human cells. However, little is known about the extent and function of lysine lactylation in fungi, here investigated with the black yeast Phialophora verrucosaverrucose as a model organism. WeHere, we report the first proteomic survey of this modification in P. verrucosa. We performed a global lactylation analysis of P. verrucosa using high accuracy bottom-up nano-LC-MS/MS in combination wwith the enrichment of lactylated peptides from digested cell lysates and subsequent peptide identification. In total, 636 lactylation sites on 420 lactylated proteins were identified in this pathogen, of which contained in 26 types of modification motifs. Our results show that over 85% of lactylated proteins were distributed in cytoplasm, mitochondria, and nucleus. The identified proteins were found to be involved associated toin diverse biological processes and were significantly enriched in the melanin biosynthesis process. Most strikingly, Kla was found in 23 structural constituent proteins of ribosome, indicating an impact of Kla in protein synthesis . Moreover, 12 lactylated proteins participated in fungal pathogenicity, suggesting a potential role for Kla in this process. Protein interaction network analysis suggested that a mass of protein interactions are regulated by lactylation. Together, our findings reveal widespread roles for lysine lactylation in regulating metabolism and melanin biosynthesis in P. verrucosa. Our data provide a rich resource for functional analyses of lactylation and facilitate the dissection of metabolic networks in this pathogen. The combined data sets represent the first report of the lactylome of P. verrucosa and provide a good foundation for further explorations of Kla in clinical fungal pathogens.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:26:13.201.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yinggai Song
SpeciesList	scientific name: Phialophora verrucosa; NCBI TaxID: 39412; scientific name: Fungi; NCBI TaxID: NCBITaxon:4751;
ModificationList	lactic acid
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-02 12:06:52	ID requested
1	2022-10-14 19:44:50	announced
⏵ 2	2023-11-14 08:26:15	announced	2023-11-14: Updated project metadata.

Song Y, Liu X, Stielow JB, de Hoog S, Li R, -related immune disorder. Front Immunol, 13():966457(2022) [pubmed]

submitter keyword: Phialophora Verrucosa, LC-MS/MS

Yinggai Song
contact affiliation	peling university first hospital
contact email	syg3515@163.com
lab head
Yinggai Song
contact affiliation	Peking University First Hospital
contact email	syg3515@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032921
     1. Label: PRIDE project
     2. Name: Post-translational changes in Phialophora verrucosa via lysine lactylation during prolonged presence in a patient with a CARD9-related immune disorder