PXD032920

PXD032920 is an original dataset announced via ProteomeXchange.

Title	Identification of vitamin K-dependent proteins in mouse liver by LC-MS/MS
Description	Vitamin K (VK), a fat-soluble vitamin, functions as a co-factor during the gamma-carboxylation reaction that converts glutamic acid residues to gamma-carboxyglutamic acid residues in proteins transiting through the endoplasmic reticulum. Two enzymes are involved which together form the VK cycle: gamma-glutamyl carboxylase (GGCX), and vitamin K oxidoreductase (VKORC1). GGCX requires reduced VK (VKH2) as an essential cofactor, which upon carboxylation, is oxidized to VK epoxide and then reconverted to VKH2 by VKORC1. Physiologically, gamma-carboxylation is essential in the liver for the activity of several coagulation factors (e.g., prothrombin, factor IX, etc.), and in arteries and cartilage to modulate the activity of Matrix Gla Protein (MGP) which prevents extra-osseous tissue mineralization. Gamma-carboxylation also negatively regulates the function of osteocalcin, a bone-derived hormone that promotes glucose homeostasis. Our objective is to identify unrecognized gamma-carboxylated proteins.
HostingRepository	PRIDE
AnnounceDate	2023-05-10
AnnouncementXML	Submission_2023-05-10_07:26:07.145.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD032920
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	DenisFaubert
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	carboxylated residue; phosphorylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-02 12:06:08	ID requested
⏵ 1	2023-05-10 07:26:07	announced

10.6019/PXD032920;

submitter keyword: gamma-carboxylation, Mouse, Liver,Vitamin K, LC-MS/MS

MathieuFerron
contact affiliation	Molecular Physiology Research Unit, Institut de Recherches Cliniques de Montréal, Montréal, Québec, Canada, H2W 1R7
contact email	mathieu.ferron@ircm.qc.ca
lab head
DenisFaubert
contact affiliation	The IRCM Mass Spectrometry and Proteomics Discovery Platform
contact email	denis.faubert@ircm.qc.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032920
     1. Label: PRIDE project
     2. Name: Identification of vitamin K-dependent proteins in mouse liver by LC-MS/MS