PXD032917

PXD032917 is an original dataset announced via ProteomeXchange.

Title	A proteomics approach towards identification and validation of blood-based protein biomarkers for the differentiation of ischemic stroke from intracerebral hemorrhage
Description	Background & aims: Stroke diagnosis is challenging in the acute phase. We aimed to determine biomarkers for the differentiation of ischemic stroke (IS) from intracerebral hemorrhage (ICH) using SWATH-MS and validate the discovered biomarkers within 24 hours using MRM proteomics. Methods: The study was conducted at Department of Neurology, All India Institute of Medical Sciences, New Delhi, India. Serum samples were collected within 24 hours from acute stroke (IS & ICH) patients & healthy controls. SWATH-MS proteomics identified significantly differentially expressed (SDE) proteins (fold change: 1.5, p<0.05 and confirmed/tentative selection using Boruta random forest) between IS and ICH which were then validated using protein MRM. Cut-off points were determined using Youden Index. Prediction models were developed using forward stepwise multivariate logistic regression analysis. Integrated discrimination improvement index determined the added value of biomarkers to clinical models. Results: Discovery phase included 20 IS, 20 ICH and 40 controls while validation phase included 150 IS and 150 ICH subjects. Total 365 proteins were quantified using SWATH-MS. Between IS and ICH, 20 SDE proteins were identified. Prediction model including clinical variables (sex, hypertension, atrial fibrillation, current smoking, baseline NIHSS) and biomarkers (GFAP, MMP9, APOC1) independently differentiated IS from ICH (accuracy: 92%, sensitivity: 96%, specificity: 69%). Addition of biomarkers improved the discrimination capacity by 26% (p<0.001) compared to clinical variables alone. Conclusion: Our study identified a range of potential protein biomarkers for the differentiation of IS. Protein biomarkers along with clinical predictors might be useful candidates in differentiating IS from ICH in acute settings.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:55:20.503.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shubham Misra
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	TSQ Altis; TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-02 12:04:54	ID requested
1	2022-09-21 10:53:37	announced
⏵ 2	2023-11-14 08:55:21	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Ischemic Stroke

Intracerebral Hemorrhage

Diagnosis

Differentiation

Blood

Biomarkers

Proteomics

Dr. Deepti Vibha
contact affiliation	Department of Neurology,Neurosciences Centre,All India Institute of Medical Sciences,Ansari Nagar, New Delhi-110029, India
contact email	deeptivibha@gmail.com
lab head
Shubham Misra
contact affiliation	All India Institute of Medical Sciences, New Delhi
contact email	shubham.misra30@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032917
     1. Label: PRIDE project
     2. Name: A proteomics approach towards identification and validation of blood-based protein biomarkers for the differentiation of ischemic stroke from intracerebral hemorrhage