PXD032906

PXD032906 is an original dataset announced via ProteomeXchange.

Title	GBPL3 is a novel plant nucleoporin that recruits transcription and RNA processing machinery at the nuclear basket by promoting condensates formation
Description	The basket of nuclear pore complex (NPC) is an assembly of flexible protein filaments and acts as a dynamic scaffold at the nucleoplasmic side that regulates various essential cellular processes, including nuclear transport, RNA processing, chromatin organization, and gene expression. However, mechanisms behind the regulation are still poorly understood in plants. Here, we identified a guanylate-binding protein (GBP)-like GTPase (GBPL3) as a specific component of the NPC basket in Arabidopsis. GBPL3 is located at the nuclear rim and confers robust physical and genetic interactions with various NPC and nucleoskeleton constituents. Proximity labeling proteomics experiment suggests that GBPL3 is predominantly distributed at the NPC basket and that it is intimately associated with chromatin remodelers, transcription regulators, and RNA processing machinery. Importantly, we found that GBPL3 promotes the formation of biomolecular condensates at nuclear periphery, which is capable of recruiting its interactors. Loss of GBPL3 led to severely stunted plant growth, aberrant transcription of abiotic and biotic stress-related genes, and retention of total mRNA in the nucleus. These results suggest that GBPL3 is a plant-specific NPC basket component and can recruit transcription and RNA processing machinery through condensate formation to modulate gene expression and subsequent RNA processing, which is required for plant growth and stress responses.
HostingRepository	PRIDE
AnnounceDate	2022-08-25
AnnouncementXML	Submission_2022-08-24_16:55:00.416.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD032906
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yu Tang
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	biotinylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2022-04-02 10:53:38	ID requested
⏵ 1	2022-08-24 16:55:00	announced

Dataset with its publication pending

submitter keyword: GBPL3, TurboID, biotin

Yangnan Gu
contact affiliation	UC Berkeley
contact email	guyangnan@berkeley.edu
lab head
Yu Tang
contact affiliation	university of California, Berkeley
contact email	yutang@berkeley.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032906
     1. Label: PRIDE project
     2. Name: GBPL3 is a novel plant nucleoporin that recruits transcription and RNA processing machinery at the nuclear basket by promoting condensates formation