PXD032881
PXD032881 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proximity-dependent proteomics identifies PARD3 scaffold protein as a novel effector in EPH receptor signaling - Part 2 |
| Description | This submission contains the mass spectrometry files for the manuscript by Sara L. Banerjee et al. that describes identification of new downstream effector proteins for EPH receptors (EPHRs). To unravel EPHR-associated signaling complexes under native conditions, we applied a mass spectrometry (MS)-based proximity labeling approach, namely BioID. We obtained a composite signaling network from EPHA4, -B2, -B3 and -B4 receptors. This network comprises 395 proteins, most of which not previously linked to EPH signaling. We examined the requirement of some of BioID-identified candidates for EPHR-regulated functions and showed that depletion of the signaling scaffold PARD3, blocks EPH-dependent cell sorting. Using affinity purification combined to MS, we further delineated a signaling complex involving C-SRC kinase (CSK), whose recruitment to PARD3 complexes is dependent on EPHR signals. The BioID and AP-MS experiments were performed from HEK293 and HEK293T cells, respectively and 30 MS files were acquired on an Orbitrap Fusion mass spectrometer in data dependent acquisition mode. All the DDA files are associated with this deposition. Please refer to the Table 1 for additional details of submitted files. For questions, please contact Jean-Philippe Lambert (Jean-Philippe.Lambert@crchudequebec.ulaval.ca) or Nicolas Bisson (nick.bisson@crchudequebec.ulaval.ca). |
| HostingRepository | MassIVE |
| AnnounceDate | 2022-06-05 |
| AnnouncementXML | Submission_2022-06-05_13:29:04.294.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Jean-Philippe Lambert |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Oxidation; deaminated residue |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-03-31 12:21:42 | ID requested | |
| ⏵ 1 | 2022-06-05 13:29:04 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: cell signaling |
Contact List
| Jean-Philippe Lambert | |
|---|---|
| contact affiliation | Universite Laval |
| contact email | jean-philippe.lambert@crchudequebec.ulaval.ca |
| lab head | |
| Jean-Philippe Lambert | |
| contact affiliation | CHU de Qu�bec-Universit� Laval Research Center |
| contact email | Jean-Philippe.Lambert@crchudequebec.ulaval.ca |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000089185/ |
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