PXD032866

PXD032866 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteins detected in HA-Mmr1 IP fraction; In vitro Mmr1 phosphorylation
Description	A capillary reverse-phase HPLC-MS/MS system was composed of a Dionex U3000 gradient pump equipped with a VICI Cheminert valve and Q Exactive equipped with a nano-electrospray ionization (NSI) source (AMR, Tokyo, Japan). The desalted peptides were loaded into a separation capillary C18 reverse-phase column (NTCC-360/100-3-125, 125×0.1 mm, Nikkyo Technos, Tokyo, Japan). Xcalibur 3.0.63 system (Thermo) was used to record peptide spectra over the mass range of m/z 350–1800. Repeatedly, MS spectra were recorded, followed by ten data-dependent high energy collisional dissociation (HCD) MS/MS spectra generated from the ten highest intensity precursor ions. MS/MS spectra were interpreted, and peak lists were generated using Proteome Discoverer 2.2.0.388 (Thermo). Searches were performed using the SEQUEST (Thermo) program against Saccharomyces cerevisiae (SwissProt TaxID = 559292) peptide sequence.
HostingRepository	jPOST
AnnounceDate	2022-03-29
AnnouncementXML	Submission_2022-09-18_03:07:45.594.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Keisuke Obara
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	S-carboxamidomethyl-L-cysteine; N6-acetyl-L-lysine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; unknown modification
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-28 21:11:09	ID requested
1	2022-03-28 21:11:12	announced
2	2022-04-17 18:08:41	announced	2022-04-18: Updated PubMed.
⏵ 3	2022-09-18 03:07:45	announced	2022-09-18: Updated FTP location.

Keyword List

submitter keyword: Yeast, PPI, In vitro phosphorylation

submitter keyword: Yeast, PPI, In vitro phosphorylation

Contact List

Takumi Kamura
lab head
Keisuke Obara
contact affiliation	Nagoya University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001546/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001546/