PXD032844 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Novel antibody-peptide binding assay indicates presence of immunoglobulins against EGFR phospho-site S1166 in high-grade glioma. |
Description | The project’s aim was to develop and test the applicability of a method to determine the presence of serum immunoglobulins against specific autoantibodies. The method combined immunoglobulin G enrichment (using Melon Gel), molecular weight filtration, and targeted mass spectrometry and the feasibility was successfully tested in an experiment using a peptide library (tryptic digest) of dinitrophenyl labelled peptides and anti-DNP. In a second step, a phospho-peptide library, derived from glioblastoma multiform tissue, was tested against plasma samples from glioma patients and healthy donors, to determine the presence of complexes of IgG with immunoaffinity to EGFR or GFAP phospho-peptides. As a result, we found immunoaffinity to EGFR phospho-peptide GSHQIS[+80]LDNPDYQQDFFPK (covering the phospho-site S1166) in plasma of glioma patients. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-07 |
AnnouncementXML | Submission_2024-10-07_13:45:58.878.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD032844 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Christoph Stingl |
SpeciesList | scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; monohydroxylated residue; 2,4-dinitrophenyl modified residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos; Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-03-27 05:48:02 | ID requested | |
⏵ 1 | 2024-10-07 13:45:59 | announced | |
Publication List
10.6019/PXD032844; |
Zeneyedpour L, Stingl C, Kros JM, Sillevis Smitt PAE, Luider TM, Novel Antibody-Peptide Binding Assay Indicates Presence of Immunoglobulins against EGFR Phospho-Site S1166 in High-Grade Glioma. Int J Mol Sci, 23(9):(2022) [pubmed] |
10.3390/ijms23095061; |
Keyword List
submitter keyword: glioblastoma, Melon Gel, peptide antigen,autoantibodies, immunoglobulin G |
Contact List
Theo M. Luider |
contact affiliation | Head of Laboratory of Neuro-Oncology, Neurology, Clinical & Cancer Proteomics |
contact email | t.luider@erasmusmc.nl |
lab head | |
Christoph Stingl |
contact affiliation | Erasmus MC Rotterdam Department of Neurology |
contact email | c.stingl@erasmusmc.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD032844
- Label: PRIDE project
- Name: Novel antibody-peptide binding assay indicates presence of immunoglobulins against EGFR phospho-site S1166 in high-grade glioma.