PXD032831

PXD032831 is an original dataset announced via ProteomeXchange.

Title	Proteome comparison of Leptospira borgpetersenii serovar Hardjo strains cultured at different temperatures
Description	Leptospirosis, caused by bacteria of the genus Leptospira, is a zoonotic disease affecting humans, companion animals, and all major livestock species. Typical propagation of the highly fastidious Leptospira borgepetesenii serovar Hardjo is limited to 29°C. However, newer culture media formulations now facilitate isolation and propagation at 37°C, a temperature that more closely emulates in vivo conditions and is hypothesized to regulate the expression of virulence factors during host infection. Since protein expression by leptospires is temperature dependent, and therefore the proteome of bacterin vaccines can differ whether grown at 37°C compared to 29°C, we compared the proteome of strains of Leptospira borgpetersenii serovar Hardjo at each temperature; two well-established strains that causes acute (strain JB197) or chronic asymptomatic disease (strain HB203) in the hamster challenge model of leptospirosis and two more recently isolated strains designated TC129 and TC273 (both of which cause chronic asymptomatic disease in the hamster). We found proteomic expression differences within strains propagated at the routine temperature of 29°C, and compared to the newly achieved culture temperature of 37°C. Results highlight significant differential protein expression, including virulence factors, amongst identical serovars of L. borgpetersenii when propagated at 29oC, the collective variation of which can be diminished when propagated at 37oC. Collectively, there is increasingly more evidence available to suggest bacterin vaccine design would benefit from consideration of strains employed, and potential effects of growth temperature related to specific behavior of pathogens in vaccine composition.
HostingRepository	PRIDE
AnnounceDate	2022-05-20
AnnouncementXML	Submission_2022-05-20_09:07:24.617.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John Lippolis
SpeciesList	scientific name: Leptospira borgpetersenii serovar Hardjo; NCBI TaxID: 328971;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-26 14:28:43	ID requested
⏵ 1	2022-05-20 09:07:25	announced

Putz EJ, Fernandes LGV, Sivasankaran SK, Bayles DO, Alt DP, Lippolis JD, Nally JE, Some like it hot, some like it cold

proteome comparison of Leptospira borgpetersenii serovar Hardjo strains propagated at different temperatures. J Proteomics, 262():104602(2022) [pubmed]

submitter keyword: Leptospirosis, Hardjo, borgpetersenii, bovine, zoonosis

Jarlath E. Nally
contact affiliation	Infectious Bacterial Disease Research Unit,USDA Agriculture Research Service,National Animal Disease Center, Ames, IA, USA.
contact email	jarlath.nally@usda.gov
lab head
John Lippolis
contact affiliation	USDA/ARS/NADC
contact email	john.lippolis@ars.usda.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032831
     1. Label: PRIDE project
     2. Name: Proteome comparison of Leptospira borgpetersenii serovar Hardjo strains cultured at different temperatures