PXD032808

PXD032808 is an original dataset announced via ProteomeXchange.

Title	Proteome of extracellular vesicles from plasma of COVID-19 patients
Description	Background: Extracellular vesicles are a valuable source of biomarkers and display the pathophysiological status of various diseases. In COVID-19, EVs are explored in several studies for their ability to reflect molecular changes caused by SARS-CoV-2 infection and impacts on disease progression. Methods: In this study, we used a label-free shotgun proteomics approach to identify and quantify any alterations in EVs proteins abundance in severe COVID-19 patients. We isolated plasma extracellular vesicles from healthy donors and patients with severe COVID-19 by size exclusion chromatography (SEC). Then, flow cytometry was performed to assess the origin of EVs and investigate the presence of circulating procoagulant EVs in COVID-19 patients. A total protein extraction was performed and samples were analyzed by nLC-MS/MS in a Q-Exactive HF-X. Finally, computational analyzes were applied to sign biological processes related to disease pathogenesis. Results: We report significant changes in the proteome of EVs from patients with severe COVID-19. Differently expressed proteins in the disease groups were associated with platelet degranulation, integrin cell surface interaction, and acute inflammatory response. Flow cytometry experiments indicated an increase in total circulating EVs and with TF-dependent procoagulant activity.
HostingRepository	PRIDE
AnnounceDate	2022-05-31
AnnouncementXML	Submission_2022-05-31_03:03:09.604.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD032808
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Monique Trugilho
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-25 05:13:09	ID requested
⏵ 1	2022-05-31 03:03:09	announced

Dataset with its publication pending

submitter keyword: Covid-19, extracellular vesicles, Proteome

Monique Trugilho
contact affiliation	Center for Technological Development in Health, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil
contact email	mrotrugilho@hotmail.com
lab head
Monique Trugilho
contact affiliation	Center for Technological Development in Health (CDTS/FIOCRUZ)
contact email	mrotrugilho@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032808
     1. Label: PRIDE project
     2. Name: Proteome of extracellular vesicles from plasma of COVID-19 patients