PXD032799

PXD032799 is an original dataset announced via ProteomeXchange.

Title	Genotype-by-environment interactions influence the composition of the Drosophila seminal proteome
Description	Ejaculate proteins are key mediators of post-mating sexual selection and sexual conflict, as they can influence both male fertilization success and female reproductive physiology. However, the extent and sources of genetic variation and condition dependence of the ejaculate proteome are largely unknown. Such knowledge could reveal the targets and mechanisms of post-mating selection and inform about the relative costs and allocation of different ejaculate components, each with its own potential fitness consequences. Here, we used liquid chromatography coupled with tandem mass spectrometry to characterize the whole-ejaculate protein composition across twelve isogenic lines of Drosophila melanogaster that were reared on a high- or low-quality diet. We discovered new proteins in the transferred ejaculate and inferred their origin in the male reproductive system. We further found that the ejaculate composition was mainly determined by genotype identity and genotype-specific responses to larval diet, with no clear overall diet effect. Nutrient restriction increased proteolytic protein activity and shifted the balance between reproductive function and RNA metabolism. Our results open new avenues for exploring the intricate role of genotypes and their environment in shaping ejaculate composition, or for studying the functional dynamics and evolutionary potential of the ejaculate in its multivariate complexity.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:01:50.464.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Valérian Zeender
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	residues isobaric at 113.084064 Da; acetylated residue; oxidized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-24 14:08:08	ID requested
1	2023-08-27 06:23:14	announced
2	2023-11-14 07:16:28	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:01:50	announced	2024-10-22: Updated project metadata.

10.1098/RSPB.2023.1313;

submitter keyword: nutrition,G×E, ejaculate protein composition., genotype

Stefan Lüpold
contact affiliation	Department of Evolutionary Biology and Environmental Studies, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
contact email	stefan.luepold@ieu.uzh.ch
lab head
Valérian Zeender
contact affiliation	University of Zurich
contact email	valerian.zeender@protonmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD032799

PRIDE project URI

• PRIDE
  1. PXD032799
     1. Label: PRIDE project
     2. Name: Genotype-by-environment interactions influence the composition of the Drosophila seminal proteome