PXD032798

PXD032798 is an original dataset announced via ProteomeXchange.

Title	Human foreskin proteome with aSTI
Description	There is scant data on the mechanisms through which asymptomatic STIs (aSTIs) alter risk of HIV acquisition in the male genital tract. Foreskin removal lowers the risk of HIV acquisition, but molecular events leading to this protection are unclear. Here we show that an asymptomatic urethral infection with Chlamydia trachomatis significantly alters the foreskin epithelial proteome composition. We developed and optimised a shotgun liquid chromatography coupled tandem mass spectrometry based proteomics approach and applied this to foreskins collected at the time of medical male circumcision from 16 aSTI+ cis-gender men and 10 age-matched STI- controls. We used a novel bioinformatic metaproteomic pipeline to detect differentially expressed proteins. Gene enrichment ontology analysis revealed proteins associated with inflammatory and immune activation function in both inner and outer foreskin from men with an aSTI. Neutrophil activation/degranulation and viral-evasion proteins were significantly enriched in foreskins from men with aSTI whereas homotypic cell-cell adhesion proteins were enriched in foreskin tissue from men without an STI. Collectively, our data show that an asymptomatic urethral sexually transmitted infection result in profound alterations in epitheial tissue that potentially causes depletion of barrier integrity and function and that may encourage HIV susceptibilty.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:42:00.688.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nyaradzo Chigorimbo-Tsikiwa
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-24 13:58:47	ID requested
1	2023-03-11 03:32:09	announced
⏵ 2	2023-11-14 08:42:01	announced	2023-11-14: Updated project metadata.

Chigorimbo-Murefu NTL, Potgieter M, Dzanibe S, Gabazana Z, Buri G, Chawla A, Nleya B, Olivier AJ, Harryparsad R, Calder B, Garnett S, Maziya L, Lewis DA, Jaspan H, Wilson D, Passmore JS, Mulder N, Blackburn J, Bekker LG, Gray CM, A pilot study to show that asymptomatic sexually transmitted infections alter the foreskin epithelial proteome. Front Microbiol, 13():928317(2022) [pubmed]

submitter keyword: MGT,Foreskin, HIV

Nyaradzo Chigorimbo-Tsikiwa
contact affiliation	Division of Medical Virology, Department of Pathology
contact email	Nyaradzo.chigorimbo-Tsikiwa@uct.ac.za
lab head
Nyaradzo Chigorimbo-Tsikiwa
contact affiliation	University of Cape Town
contact email	Nyaradzo.chigorimbo-tsikiwa@uct.ac.za
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD032798

PRIDE project URI

• PRIDE
  1. PXD032798
     1. Label: PRIDE project
     2. Name: Human foreskin proteome with aSTI