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PXD032788

PXD032788 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLabeled quantitative proteomics dataset of optogenetics induced axon regeneration
DescriptionThis labeled quantitative proteomics dataset was collected from a transgenic channel rhodopsin mouse model (Chr2) subjected to light stimulation after traumatic optic nerve crush (ONC) was performed. Mouse models expressing channel rhodopsin were subjected to therapeutic light stimulation promoting axon regeneration of retinal ganglion cell (RGC) axons post optic nerve crush. Experimental mice and wild type control mice were euthanized, and optic nerves were collected. A protein extraction was carried out by careful mincing of the tissue in extraction buffer (TEAB, NaCl and SDS). Protein amount normalization across samples was achieved using dot blot densitometry using ImageJ software. Samples were labelled using a modified 16plex TMT (Tandem Mass Tag) kit for quantification after overnight trypsin digestion. Untargeted liquid chromatography-mass spectrometry was performed on an Easy-nLC 1000 liquid chromatograph coupled to a QExactive mass spectrometer (LC-MS/MS). Data analysis was performed using Proteome Discoverer 2.5.
HostingRepositoryPRIDE
AnnounceDate2022-08-12
AnnouncementXMLSubmission_2022-08-12_00:06:46.377.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD032788
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterSanjoy Bhattacharya
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-03-24 13:29:49ID requested
12022-08-12 00:06:47announced
Publication List
Harvey FC, Mendoza X, Liu Y, Lee RK, Bhattacharya SK, Labeled quantitative proteomics dataset of optogenetics induced axon regeneration in mice. Data Brief, 42():108304(2022) [pubmed]
Keyword List
submitter keyword: Axon regeneration, optogenetic stimulation, rhythmic light stimulation, quantitative proteome
Contact List
Sanjoy K Bhattacharya
contact affiliationUniversity of Miami Miller School of Medicine, Department of Ophthalmology, Miami Integrative Metabolomics Research Center, Bascom Palmer Eye Institute (McKnight Vision Research Center)
contact emailsbhattacharya@med.miami.edu
lab head
Sanjoy Bhattacharya
contact affiliationBascom Palmer Eye Institute, Department of Ophthalmology, Miller School of Medicine at University of Miami, Miami, FL 33136, USA
contact emailsbhattacharya@med.miami.edu
dataset submitter
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Dataset FTP location
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