PXD032777

PXD032777 is an original dataset announced via ProteomeXchange.

Title	Proteomic studies of the mechanism of cytotoxicity, induced by palytoxin on HaCaT cells
Description	Palytoxin (PLTX) is a polyether Marine toxin isolated from sea anemones. It is one of the most toxic non-protein substances, causing many people to be poisoned every year and die in severe cases. With its known impact on Na+, K+-ATPase, much still remain unclear about the mechanism of PLTX action. Here, we tested different concentrations of PLTX on HaCaT cells, and studied its distributions in cells, its impact on gene expressions, and associated pathways using proteomics combined with bioinformatics tools. We found that PLTX could cause ferroptosis in HaCaT cells, a new type of programmed cell death, by up-regulating the expressions of VDAC3, ACSL4, and NCOA4 that lead to the occurrence of ferroptosis. PLTX also acts on MAPK pathway that is related to cell apoptosis, proliferation, division and differentiation. Different from that of ferroptosis, PLTX down-regulates the expression of ERK, and as a result, the expressions of MAPK1, MAP2K1 and MAP2K2 are also lower which will affect cell proliferation. The genes from these two mechanisms showed interactions but we didn’t find overlap genes between the two. Both ferroptosis and MAPK pathways can be used as anticancer targets, so PLTX may become an anticancer drug with appropriate modification.
HostingRepository	iProX
AnnounceDate	2022-03-24
AnnouncementXML	Submission_2022-09-29_01:25:59.999.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dingyuan Cheng
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-23 20:50:54	ID requested
1	2022-03-23 20:51:34	announced
⏵ 2	2022-09-29 01:26:00	announced	2022-09-29: Update publication information.

Cheng D, Deng B, Tong Q, Gao S, Xiao B, Zhu M, Ren Z, Wang L, Sun M, Proteomic Studies of the Mechanism of Cytotoxicity, Induced by Palytoxin on HaCaT Cells. Toxins (Basel), 14(4):(2022) [pubmed]

submitter keyword: Palytoxin, Proteomic Analysis, MAPK, Ferroptosis

Minjuan Sun
contact affiliation	Naval Medical University
contact email	sunmj@smmu.edu.cn
lab head
Dingyuan Cheng
contact affiliation	Navy medical University
contact email	674730032@qq.com
dataset submitter

iProX dataset URI