PXD032763

PXD032763 is an original dataset announced via ProteomeXchange.

Title	- The STAR assay: A new sensitive, selective, and versatile method for isolation of DNA-protein crosslinks
Description	- DNA-protein crosslinks (DPCs), formed by covalent conjugation of proteins to DNA molecule, are toxic DNA lesions created by exposure to physical or chemical insults or faulty enzymatic reactions. Because of their bulky nature and chemical changes to DNA molecule, they interfere with DNA metabolic processing and transcription. With the recent discovery of proteases and nucleases that participate in the repair of such lesions, the development of an accurate biochemical assay for DPC isolation became a priority for further mechanistic understanding of their repair. Here we propose the STAR assay, a novel sensitive, selective, and versatile method for the direct quantification of DPCs, with high sensitivity to physiologically relevant treatment conditions. The assay is efficient in detecting physically, chemically, or enzymatically created adducts. We demonstrate that nuclear localization is required for crosslinking the proteins to DNA molecule. Combining the STAR assay results with Comet assay and western blot confirmed that the repair of DPCs is a two-step process, starting with the removal of protein part of the lesions followed by the repair of the DNA molecule itself. The STAR assay is applicable for studying formation, repair, and biological significance of DPCs, as well as for chemotherapeutics dose management in personalized medicine.
HostingRepository	PRIDE
AnnounceDate	2023-01-17
AnnouncementXML	Submission_2023-01-17_05:04:00.464.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	NicolasNalpas
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-23 08:12:46	ID requested
⏵ 1	2023-01-17 05:04:00	announced

Dataset with its publication pending

submitter keyword: DNA-adduct repair, DNA damage repair, Topoisomerase inhibitors,- DNA-protein crosslinks, DPCs, Formaldehyde

Prof. Ivana MarinovićTerzić
contact affiliation	University of Split School of Medicine Laboratory for cancer research Soltanska 2 21 000 Split Croatia
contact email	ivana.marinovic.terzic@mefst.hr
lab head
NicolasNalpas
contact affiliation	Tuebingen University
contact email	nalpas.nicolas@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD032763

PRIDE project URI

• PRIDE
  1. PXD032763
     1. Label: PRIDE project
     2. Name: - The STAR assay: A new sensitive, selective, and versatile method for isolation of DNA-protein crosslinks