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PXD032754

PXD032754 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleShort and mid term heat stress proteomics characterization in pinus radiata
DescriptionIn order to study in detail short and mid term heat stress molecular response we performed a shotgun proteomics study. One-year-old P. radiata seedlings (plant size ∼33 ± 4 cm) were kept in 1 dm3 pots under a photoperiod of 16 h (400 µmol m−2 s−1) at 25 °C and 50% relative humidity (RH), and 15 °C and 60% RH during the night period. The plants were previously acclimated over a 1-month period inside the climate chamber, being watered with nutritive solution (N : P : K, 5 : 8 : 10). In order to perform a realistic experiment, and corresponding with the dawn, heat exposure treatment began with an increasing temperature gradient from 15 to 40 °C over 5 h, which was then maintained for 6 h; after that, temperature was gradually decreased from 40 to 15 °C over 5 h. This experimental procedure was repeated for 5 days and sampling was performed at: 3 h after 40 °C was reached on Day 1 (T1/2) and at the end of the 6 h heat exposure on Day 1 (T1), Day 2 (T2), Day 3 (T3) and Day 5 (T5) (Figure 1a). Plants were watered every day to 80% full capacity (FC) in order to avoid a collateral drought stress. A group of control plants was also collected on Day 1 (C) before starting the heat exposure.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:41:45.629.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLaura Lamelas
SpeciesList scientific name: Pinus radiata (Monterey pine); NCBI TaxID: 3347;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-03-23 07:58:25ID requested
12023-03-11 03:25:13announced
22023-11-14 08:41:46announced2023-11-14: Updated project metadata.
Publication List
Roces V, Lamelas L, Valledor L, Carb, ó M, Ca, ñ, al MJ, Meij, ó, n M, Integrative analysis in Pinus revealed long-term heat stress splicing memory. Plant J, 112(4):998-1013(2022) [pubmed]
Keyword List
submitter keyword: pine, orbitrap, climate change
Contact List
Mónica Meijón
contact affiliationPlant Physiology, department of Organisms and Systems Biology, University of Oviedo, Spain
contact emailmeijonmonica@uniovi.es
lab head
Laura Lamelas
contact affiliationUniversidad de Oviedo
contact emaillamelaslaura@uniovi.es
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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