PXD032754 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Short and mid term heat stress proteomics characterization in pinus radiata |
Description | In order to study in detail short and mid term heat stress molecular response we performed a shotgun proteomics study. One-year-old P. radiata seedlings (plant size ∼33 ± 4 cm) were kept in 1 dm3 pots under a photoperiod of 16 h (400 µmol m−2 s−1) at 25 °C and 50% relative humidity (RH), and 15 °C and 60% RH during the night period. The plants were previously acclimated over a 1-month period inside the climate chamber, being watered with nutritive solution (N : P : K, 5 : 8 : 10). In order to perform a realistic experiment, and corresponding with the dawn, heat exposure treatment began with an increasing temperature gradient from 15 to 40 °C over 5 h, which was then maintained for 6 h; after that, temperature was gradually decreased from 40 to 15 °C over 5 h. This experimental procedure was repeated for 5 days and sampling was performed at: 3 h after 40 °C was reached on Day 1 (T1/2) and at the end of the 6 h heat exposure on Day 1 (T1), Day 2 (T2), Day 3 (T3) and Day 5 (T5) (Figure 1a). Plants were watered every day to 80% full capacity (FC) in order to avoid a collateral drought stress. A group of control plants was also collected on Day 1 (C) before starting the heat exposure. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:41:45.629.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Laura Lamelas |
SpeciesList | scientific name: Pinus radiata (Monterey pine); NCBI TaxID: 3347; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-03-23 07:58:25 | ID requested | |
1 | 2023-03-11 03:25:13 | announced | |
⏵ 2 | 2023-11-14 08:41:46 | announced | 2023-11-14: Updated project metadata. |
Publication List
Roces V, Lamelas L, Valledor L, Carb, ó M, Ca, ñ, al MJ, Meij, ó, n M, Integrative analysis in Pinus revealed long-term heat stress splicing memory. Plant J, 112(4):998-1013(2022) [pubmed] |
Keyword List
submitter keyword: pine, orbitrap, climate change |
Contact List
Mónica Meijón |
contact affiliation | Plant Physiology, department of Organisms and Systems Biology, University of Oviedo, Spain |
contact email | meijonmonica@uniovi.es |
lab head | |
Laura Lamelas |
contact affiliation | Universidad de Oviedo |
contact email | lamelaslaura@uniovi.es |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD032754 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD032754
- Label: PRIDE project
- Name: Short and mid term heat stress proteomics characterization in pinus radiata