PXD032739

PXD032739 is an original dataset announced via ProteomeXchange.

Title	Characterization vy crosslinking-mass spectrometry of plastid-encoded RNA polymerase purified from Sinapis alba.
Description	RNA polymerases (RNAPs) transcribe DNA into RNA and are found in all living organisms with several degrees of complexity from single polypeptide chain to multimeric enzymes. In chloroplast of angiosperms, two RNAPs are involved in plastid gene transcription: the nuclear-encoded RNA polymerase (NEP) and the plastid-encoded RNA polymerase (PEP). The PEP is a prokaryotic-type multimeric RNAP found in different states depending on light stimuli and cell identity. One of these active states requires the assembly of nuclear-encoded proteins named PEP-Associated Proteins (PAPs) with the catalytic core, triggering the timely transcription of photosynthesis-associated plastid genes in cells acquiring their photosynthetic apparatus. A purification procedure was used to enrich native PEP from Sinapis alba chloroplasts. Crosslinking coupled to mass spectrometry provided initial structural information about the relative position of PEP subunits within the complex.
HostingRepository	PRIDE
AnnounceDate	2022-09-12
AnnouncementXML	Submission_2022-09-12_00:06:21.989.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: Sinapis alba; NCBI TaxID: 3728;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-23 07:26:29	ID requested
⏵ 1	2022-09-12 00:06:22	announced

Ruedas R, Muthukumar SS, Kieffer-Jaquinod S, Gillet FX, Fenel D, Effantin G, Pfannschmidt T, Cout, é Y, Blanvillain R, Cobessi D, . Int J Mol Sci, 23(17):(2022) [pubmed]

submitter keyword: plastid-encoded RNA polymerase, PEP associated proteins, transcription, photomorphogenesis, photosynthesis, chloroplast biogenesis, crosslinking, nanoLC-MS/MS

Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
lab head
Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032739
     1. Label: PRIDE project
     2. Name: Characterization vy crosslinking-mass spectrometry of plastid-encoded RNA polymerase purified from Sinapis alba.