PXD032738 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mass spectrometry-based proteomic characterization of plastid-encoded RNA polymerase purified from Sinapis alba. |
Description | RNA polymerases (RNAPs) transcribe DNA into RNA and are found in all living organisms with several degrees of complexity from single polypeptide chain to multimeric enzymes. In chloroplast of angiosperms, two RNAPs are involved in plastid gene transcription: the nuclear-encoded RNA polymerase (NEP) and the plastid-encoded RNA polymerase (PEP). The PEP is a prokaryotic-type multimeric RNAP found in different states depending on light stimuli and cell identity. One of these active states requires the assembly of nuclear-encoded proteins named PEP-Associated Proteins (PAPs) with the catalytic core, triggering the timely transcription of photosynthesis-associated plastid genes in cells acquiring their photosynthetic apparatus. A purification procedure was used to enrich native PEP from Sinapis alba chloroplasts. Mass spectrometry-based proteomic analysis of the obtained fraction identified the expected subunits, i.e. the core components and the PAPs as the most abundant proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2022-09-12 |
AnnouncementXML | Submission_2022-09-12_00:06:26.861.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD032738 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Yohann Couté |
SpeciesList | scientific name: Sinapis alba; NCBI TaxID: 3728; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-03-23 07:26:08 | ID requested | |
⏵ 1 | 2022-09-12 00:06:27 | announced | |
Publication List
Ruedas R, Muthukumar SS, Kieffer-Jaquinod S, Gillet FX, Fenel D, Effantin G, Pfannschmidt T, Cout, é Y, Blanvillain R, Cobessi D, . Int J Mol Sci, 23(17):(2022) [pubmed] |
Keyword List
submitter keyword: plastid-encoded RNA polymerase, PEP associated proteins, transcription, photomorphogenesis, photosynthesis, chloroplast biogenesis, nanoLC-MS/MS |
Contact List
Yohann Couté |
contact affiliation | EDyP |
contact email | yohann.coute@cea.fr |
lab head | |
Yohann Couté |
contact affiliation | EDyP |
contact email | yohann.coute@cea.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD032738
- Label: PRIDE project
- Name: Mass spectrometry-based proteomic characterization of plastid-encoded RNA polymerase purified from Sinapis alba.