PXD032736

PXD032736 is an original dataset announced via ProteomeXchange.

Title	Application of Two-Dimensional Difference Gel Electrophoresis to Identify Protein Changes between Center, Margin, and Adjacent Non-Tumor Tissues Obtained from Non-Small-Cell Lung Cancer with Adenocarcinoma Subtype
Description	Lung cancer is responsible for the most cancer-related mortality worldwide and the mechanism of its development is poorly understood. Proteomics has become a powerful tool offering vital knowledge related to cancer development. Using a two-dimensional difference gel electrophoresis (2D-DIGE) approach, we sought to compare tissue samples from non-small-cell lung cancer (NSCLC) patients, exactly adenocarcinoma (ADC), taken from the tumor center and tumor margin and control (adjacent non-tumor) tissues . We found 22 differentially abundant spots representing 17 proteins differentiating center and margin. Sixty eight proteins; represented by 79 spots :40, 20, and 19 spots differed between the control and center and margin, control and center, and control and margin, respectively. Twenty six significant canonical pathways were identified, including Rho signaling pathways, a semaphorin neuronal repulsive signaling pathway, and epithelial adherens junction signaling. Proteins differentiating the tumor center and tumor margin were linked to cancer invasion and progression, including cell migration, adhesion and invasion, cytoskeletal structure, protein folding, anaerobic metabolism, tumor angiogenesis, EMC transition, epithelial adherens junctions, and inflammatory responses.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:39:40.507.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mariola Dietrich
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	autoflex TOF/TOF; autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-23 07:23:52	ID requested
⏵ 1	2024-10-08 11:39:45	announced

10.1371/journal.pone.0268073;

Ciereszko A, Dietrich MA, S, ł, owi, ń, ska M, Nynca J, Ciborowski M, Kaczmarek MM, Myszczy, ń, ski K, Ki, ś, luk J, Majewska A, Michalska-Falkowska A, Kodzik N, Resze, ć J, Sierko E, Nikli, ń, ski J, Application of two-dimensional difference gel electrophoresis to identify protein changes between center, margin, and adjacent non-tumor tissues obtained from non-small-cell lung cancer with adenocarcinoma or squamous cell carcinoma subtype. PLoS One, 17(5):e0268073(2022) [pubmed]

submitter keyword: cancer, lung tissue,human, 2D-DIGE, adenocarcinoma

Mariola Dietrich
contact affiliation	Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences, Olsztyn, 10-748, Poland
contact email	m.dietrich@pan.olsztyn.pl
lab head
Mariola Dietrich
contact affiliation	Polish Academy of Sciences
contact email	m.dietrich@pan.olsztyn.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032736
     1. Label: PRIDE project
     2. Name: Application of Two-Dimensional Difference Gel Electrophoresis to Identify Protein Changes between Center, Margin, and Adjacent Non-Tumor Tissues Obtained from Non-Small-Cell Lung Cancer with Adenocarcinoma Subtype