PXD032733

PXD032733 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomic Analysis Reveals potential roles of PRDX3 for neurite outgrowth in N2a-APPswe Cells
Description	Alzheimer’s disease (AD) is characterized by amyloid plaques and neurofibrillary tangles accompanied with progressive neurite loss. Mitochondria play pivotal roles in Alzheimer’s disease development. PRDX3 is a mitochondrial peroxide reductase responsible for H2O2 scavenge and signaling transduction. In this study, we found knockdown PRDX3 in N2a-APPSwe cell line induced neurite outgrowth, but not reduced cell viability against tert-butyl hydroperoxide (TBHP) induced cell damage. We found knockdown of PRDX3 induced dysregulation of one hundred proteins by Tandem Mass Tag (TMT) labeled proteomic study. Gene Ontology analysis revealed that the dysregulated proteins are enriched on establishment of protein localization to plasma membrane, lipid catabolic process, and intermediate filament cytoskeleton organization. String analysis showed closely protein-protein interactions among the dysregulated proteins. Annexin A1 (ANXA1), serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform (PP2A) and glutathione S-transferase Mu 2 (GSTM2) were significantly upregulated in PRDX3 knockdown N2a-APPswe cell lines and verified by western blot. Our study for the first time revealed PRDX3 may also played important roles on neurite outgrowth and related AD development.
HostingRepository	PRIDE
AnnounceDate	2022-03-24
AnnouncementXML	Submission_2022-03-23_18:18:47.840.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD032733
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Benhong Xu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-23 07:13:01	ID requested
⏵ 1	2022-03-23 18:18:48	announced

Xu B, Gao C, Zhang H, Huang X, Yang X, Yang C, Liu W, Wu D, Liu J, A quantitative proteomic analysis reveals the potential roles of PRDX3 in neurite outgrowth in N2a-APPswe cells. Biochem Biophys Res Commun, 604():144-150(2022) [pubmed]

submitter keyword: mitochondria,PRDX3, neurite

Benhong Xu
contact affiliation	Shenzhen Center for Disease Control and Prevention
contact email	xubenhong@szcdc.net
lab head
Benhong Xu
contact affiliation	Shenzhen Center for Disease Control and Prevention
contact email	xubenhong@szcdc.net
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032733
     1. Label: PRIDE project
     2. Name: Quantitative Proteomic Analysis Reveals potential roles of PRDX3 for neurite outgrowth in N2a-APPswe Cells