PXD032731

PXD032731 is an original dataset announced via ProteomeXchange.

Title	Development of functional anti-Gn nanobodies specific for SFTSV based on next generation sequencing and proteomics
Description	Severe fever with thrombocytopenia syndrome (SFTS) is an acute infectious disease caused by novel bunyavirus (SFTSV), with a mortality rate of 6.3% ~ 30%. To date, there is no specific treatment for SFTS. Previously, our studies demonstrate that SFTSV surface glycoprotein (Glycoprotein N, Gn) is a potential target for the development of SFTS vaccine or therapeutic antibodies, and anti-Gn neutralizing antibodies play a protective role in SFTS infection. Compared with traditional antibodies, nanobodies from camelids have various advantages including small molecular weight, high affinity, low immunogenicity and convenient production by gene engineering, etc. In this study, we combined next generation sequencing (NGS) with proteomics technology and bioinformatics analysis to high-throughput screen monoclonal anti-Gn nanobodies from camel immunized with Gn protein. We identified 19 anti-Gn monoclonal nanobody sequences, and selected 6 of them for recombinant protein expression and purification. Among these 6 anti-Gn nanobodies, nanobody 57493 was validated to be highly specific for Gn.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_06:27:16.780.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lei Fang
SpeciesList	scientific name: Camelidae; NCBI TaxID: 9835;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-23 07:12:15	ID requested
⏵ 1	2024-01-26 06:27:17	announced

Wang B, Huang B, Li X, Guo Y, Qi G, Ding Y, Gao H, Zhang J, Wu X, Fang L, Development of functional anti-Gn nanobodies specific for SFTSV based on next-generation sequencing and proteomics. Protein Sci, 31(11):e4461(2022) [pubmed]

10.1002/pro.4461;

submitter keyword: LC MS/MS, Nanobody,Camel

Lei Fang
contact affiliation	Medical School of Nanjing University, China
contact email	njfanglei@nju.edu.cn
lab head
Lei Fang
contact affiliation	Nanjing University Medical School
contact email	njfanglei@nju.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD032731

PRIDE project URI

• PRIDE
  1. PXD032731
     1. Label: PRIDE project
     2. Name: Development of functional anti-Gn nanobodies specific for SFTSV based on next generation sequencing and proteomics