PXD032729

PXD032729 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of skeletal muscle from the Hybryid Mouse Diversity Panel
Description	To begin to understand how variations in the genome drive changes in the skeletal muscle proteome, we performed a proteomic analysis of Gastrocnemius muscle from 73 inbred mouse strains of the Hybrid Mouse Diversity Panel (HMDP) that were fed a chow diet and housed under identical environmental conditions (n=2-4; 162 mice). These data were integrated with genomic and various molecular/phenotypic data via systems genetic analysis which is a powerful forward genetics approach to identify potential new regulators of complex traits. Proteomics was performed with eighteen 10-plex tandem mass tags (TMT) experiments each consisting of 9 strains plus a pooled common internal reference. Peptides were analysed by 2D-liquid chromatography coupled to tandem mass spectrometry (2D-LC-MS/MS) resulting in the quantification of 5,350 proteins with 4,027 quantified in >50 mice and 2,069 proteins quantified in all 162 animals.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:40:36.854.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benjamin Parker
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-23 07:11:20	ID requested
1	2023-01-08 16:37:02	announced
⏵ 2	2023-11-14 07:40:37	announced	2023-11-14: Updated project metadata.

Molendijk J, Blazev R, Mills RJ, Ng YK, Watt KI, Chau D, Gregorevic P, Crouch PJ, Hilton JBW, Lisowski L, Zhang P, Reue K, Lusis AJ, Hudson JE, James DE, Seldin MM, Parker BL, Proteome-wide systems genetics identifies UFMylation as a regulator of skeletal muscle function. Elife, 11():(2022) [pubmed]

submitter keyword: hybrid mouse diversity panel,skeletal muscle

Benjamin Parker
contact affiliation	The University of Melbourne
contact email	ben.parker@unimelb.edu.au
lab head
Benjamin Parker
contact affiliation	The University of Melbourne
contact email	ben.parker@unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD032729

PRIDE project URI

• PRIDE
  1. PXD032729
     1. Label: PRIDE project
     2. Name: Proteomic analysis of skeletal muscle from the Hybryid Mouse Diversity Panel