PXD032728

PXD032728 is an original dataset announced via ProteomeXchange.

Title	Analysis of subcellular Importin b1 interactomes by BioID and BAR proximity labelling
Description	Importin 1 (KPNB1) is a nucleocytoplasmic transport factor with critical roles in both cytoplasmic and nucleocytoplasmic transport, hence there is keen interest in the characterization of its subcellular interactomes. We found limited efficiency of BioID in detection of importin complex cargos, and therefore generated a highly specific and sensitive anti-KPNB1 monoclonal antibody to enable Biotinylation by Antibody Recognition (BAR) analysis of Importin 1 interactomes.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:24:15.967.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juan Oses-Prieto
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-23 07:05:44	ID requested
1	2022-10-14 19:32:20	announced
⏵ 2	2023-11-14 08:24:16	announced	2023-11-14: Updated project metadata.

Song DA, Alber S, Doron-Mandel E, Schmid V, Albus CA, Leitner O, Hamawi H, Oses-Prieto JA, Dezorella N, Burlingame AL, Fainzilber M, Rishal I, 1 Interactomes. Mol Cell Proteomics, 21(11):100418(2022) [pubmed]

submitter keyword: Axonal transport, KPNB1, BAR, BioID,Proximity labeling

Mike Fainzilber
contact affiliation	1Departments of Biomolecular Sciences and Molecular Neuroscience, Weizmann Institute of Science, 7610001 Rehovot, Israel
contact email	mike.fainzilber@weizmann.ac.il
lab head
Juan Oses-Prieto
contact affiliation	UCSF
contact email	joses@cgl.ucsf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD032728

PRIDE project URI

• PRIDE
  1. PXD032728
     1. Label: PRIDE project
     2. Name: Analysis of subcellular Importin b1 interactomes by BioID and BAR proximity labelling