PXD032706
PXD032706 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Modern Metaproteomics: A Unique Tool to Characterize the Active Microbiome in Health and Diseases, and Pave the Road towards New Biomarkers. Example of Crohn's Disease and Ulcerative Colitis Flare-ups |
| Description | Thanks to the latest developments in mass spectrometry, software and standards, metaproteomics is emerging as the vital complement of metagenomics, to make headway in understanding the actual functioning of living and active microbial communities. Modern metaproteomics offers new possibilities in the area of clinical diagnosis. This is illustrated here, for the still highly challenging diagnosis of intestinal bowel diseases (IBDs). Using bottom-up proteomics, we analyzed the gut metaproteomes of the same twenty fecal specimens processed either fresh or after a two-month freezing period. We focused on metaproteomes of microbial cell envelopes since it is an outstanding way of capturing host and host-microbe interaction signals. The protein profiles of pairs of fresh and frozen-thawed samples were closely related, making feasible deferred analysis in a distant diagnosis centre. The taxonomic and functional landscape of microbes in diverse IBD phenotypes active ulcerative colitis, or active Crohn's disease either with ileo-colonic or exclusive colonic localization differed from each other and from the controls. Based on their specific peptides, we could identify proteins that were either strictly overrepresented or underrepresented in all samples of one clinical group compared to all samples of another group, paving the road for promising new diagnosis tools of IBD |
| HostingRepository | MassIVE |
| AnnounceDate | 2022-11-22 |
| AnnouncementXML | Submission_2022-11-22_09:25:28.657.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | HENRY |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Carbamidomethyl; Oxidation |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-03-22 12:09:23 | ID requested | |
| ⏵ 1 | 2022-11-22 09:25:29 | announced |
Publication List
| Henry C, Bassignani A, Berland M, Langella O, Sokol H, Juste C, Modern Metaproteomics: A Unique Tool to Characterize the Active Microbiome in Health and Diseases, and Pave the Road towards New Biomarkers-Example of Crohn's Disease and Ulcerative Colitis Flare-Ups. Cells, 11(8):(2022) [pubmed] |
Keyword List
| submitter keyword: metaproteomics, mass spectrometry, biomarkers, Crohn's disease, ulcerative colitis |
Contact List
| celine Henry | |
|---|---|
| contact affiliation | INRAE |
| contact email | celine.henry@inrae.fr |
| lab head | |
| HENRY | |
| contact affiliation | INRAE |
| contact email | celine.henry@inrae.fr |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000089099/ |
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