PXD032689

PXD032689 is an original dataset announced via ProteomeXchange.

Title	TMT-based quantitative membrTMT-based quantitative membrane proteomics reveals in-depth investigation of PRRs involved in the perception of MSP1 in rice leavesane proteomics reveals in-depth investigation of PRRs involved in the perception of MSP1 in rice leaves
Description	Pathogen secreted pathogen-associated molecular patterns (PAMPs) play a key role in PAMPs triggered immunity (PTI) in plant for the recognition of pathogens. In rice, fewer PAMPs and their pattern recognition receptors (PRRs) have been characterized during rice-Magnaporthe oryzae interaction. Particularly, recent study was identified M. oryzae snodprot1 homolog known as MSP1, however, the molecular mechanism of MSP1 induced PTI is currently elusive. Therefore, we were generated the MSP1 overexpressed transgenic rice with their subcellular localization in the apoplastic (with signal sequence) and cytoplasmic (without signal sequence) regions, respectively, to examine the functional role of MSP1 in rice. Here, we employed a tandem mass tag (TMT)-based quantitative membrane proteomic analysis to decipher the potential interaction PRRs and MSP1-induced downstream signaling. This approach led to the identification of 8,033 proteins and sequential statistical analysis were identified 2,226 differentially modulated proteins. Of these, 20 plasma membrane localized receptor like kinases (RLKs) with the increased abundance in response to MSP1. Moreover, activation of proteins related to the protein degradation and modification, calcium signaling, transcription factor, redox, and MAPK signaling were characterized. Taken together, our results indicated that potential PRR candidates involved in response to blast disease and thus suggesting the overview mechanism of the MSP1-induced signaling in rice leaves.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-12_10:15:52.164.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sun Tae Kim
SpeciesList	scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-21 08:40:11	ID requested
⏵ 1	2022-08-12 10:15:52	announced

Min CW, Jang JW, Lee GH, Gupta R, Yoon J, Park HJ, Cho HS, Park SR, Kwon SW, Cho LH, Jung KH, Kim YJ, Wang Y, Kim ST, TMT-based quantitative membrane proteomics identified PRRs potentially involved in the perception of MSP1 in rice leaves. J Proteomics, 267():104687(2022) [pubmed]

submitter keyword: Rice, Magnaporthe oryzae, MSP1, TMT, PRRs, MME method, Plant-pathogen interaction

Sun Tae Kim
contact affiliation	Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang, 50463, Republic of Korea
contact email	stkim5505@gmail.com
lab head
Sun Tae Kim
contact affiliation	Pusan National University
contact email	stkim5505@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032689
     1. Label: PRIDE project
     2. Name: TMT-based quantitative membrTMT-based quantitative membrane proteomics reveals in-depth investigation of PRRs involved in the perception of MSP1 in rice leavesane proteomics reveals in-depth investigation of PRRs involved in the perception of MSP1 in rice leaves