PXD032677

PXD032677 is an original dataset announced via ProteomeXchange.

Title	MultiOMICs landscape of SARS-CoV2-induced host responses in human lung epithelial cells
Description	COVID-19 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection continues to be a global health pandemic. Despite the availability of vaccines and approved therapeutics, the emergence of newer plausible resistant variants continues to be on the rise. Several multi-omics studies have provided extensive evidence of host-pathogen interactions and potential therapeutic targets. However, an increased understanding of host cellular signaling networks regulated by multiple post-translational modifications and its ensuing effect on the biochemical and cellular dynamics is vital to expand the current knowledge on the host response to SARS-CoV-2 infections. Here, employing unbiased global proteomics, acetylomics, phosphoproteomics, RNA sequencing, and exometabolome analysis of a lung-derived human cell line, we show that SARS-CoV2 Norway/Trondheim-S15 strain induces time-dependent alterations in host cellular processes, including induction of type I IFN response, activation of DNA damage response pathway, RNA processing, metabolism, and cytoskeletal reorganization. We provide evidence for the interplay of phosphorylation and acetylation dynamics on host proteins and its effect on the altered release of metabolites, especially organic acids and ketone bodies. Together, our findings can serve as a resource for the identification of potential targets to design newer host-directed therapeutic strategies.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:03:32.394.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard K. Kandasamy
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-20 15:44:22	ID requested
1	2023-01-18 12:54:22	announced
2	2023-01-25 13:25:28	announced	2023-01-25: Updated project metadata.
⏵ 3	2023-11-14 08:03:34	announced	2023-11-14: Updated project metadata.

Pinto SM, Subbannayya Y, Kim H, Hagen L, G, ó, rna MW, Nieminen AI, Bj, ø, r, å, s M, Espevik T, Kainov D, Kandasamy RK, Multi-OMICs landscape of SARS-CoV-2-induced host responses in human lung epithelial cells. iScience, 26(1):105895(2023) [pubmed]

ProteomeXchange project tag: Sars-cov-2, Covid-19

submitter keyword: SARS-CoV-2, inflammation, multi-OMICS, innate immune signaling, functional networks, COVID-19, signaling

Richard K. Kandasamy
contact affiliation	Systems Inflammation group, Centre of Molecular Inflammation Research (CEMIR), and Department of Clinical and Molecular Medicine (IKOM), Norwegian University of Science and Technology, Norway
contact email	richard.k.kandasamy@ntnu.no
lab head
Richard K. Kandasamy
contact affiliation	NTNU
contact email	richard.k.kandasamy@ntnu.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD032677

PRIDE project URI

• PRIDE
  1. PXD032677
     1. Label: PRIDE project
     2. Name: MultiOMICs landscape of SARS-CoV2-induced host responses in human lung epithelial cells