PXD032675

PXD032675 is an original dataset announced via ProteomeXchange.

Title	Profiling HeLa cell ubiquitylome with OtUBD and FK2
Description	This project compares the novel ubiquitin affinity reagent OtUBD with a monoclonal ubiquitin antibody FK2 in their abilities to facilitae the profiling of the ubiquitylated proteome of HeLa cells. Enrichments were done side by side using a semi-denaturing condition.
HostingRepository	PRIDE
AnnounceDate	2022-05-30
AnnouncementXML	Submission_2022-05-30_06:16:26.800.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mengwen Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	L-methionine sulfoxide; ubiquitinylated lysine; methylthiolated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-20 15:43:41	ID requested
⏵ 1	2022-05-30 06:16:27	announced

Dataset with its publication pending

submitter keyword: UBD, HeLa cells, LC-MS, ubiquitin

Mark Hochstrasser
contact affiliation	Yale University
contact email	mark.hochstrasser@yale.edu
lab head
Mengwen Zhang
contact affiliation	Yale University
contact email	mengwen.zhang@yale.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD032675

PRIDE project URI

• PRIDE
  1. PXD032675
     1. Label: PRIDE project
     2. Name: Profiling HeLa cell ubiquitylome with OtUBD and FK2