PXD032663 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The dopamine transporter antiports potassium to increase the uptake of dopamine |
Description | The dopamine transporter facilitates dopamine reuptake from the extracellular space to terminate neurotransmission. The transporter belongs to the neurotransmitter:sodium symporter family, which includes transporters for serotonin, norepinephrine, and GABA that utilize the Na+ gradient to drive the uptake of substrate. Decades ago, it was shown that the serotonin transporter also antiports K+, but investigations of K+-coupled transport in other neurotransmitter:sodium symporters have been inconclusive. Here, we show that ligand binding to the drosophila- and human dopamine transporters are inhibited by K+, and the conformational dynamics of the drosophila dopamine transporter in K+ are divergent from the apo- and Na+-states. Furthermore, we found that K+ increased dopamine uptake by the drosophila dopamine transporter in liposomes, and visualized Na+ and K+ fluxes in single proteoliposomes using fluorescent ion indicators. Our results expand on the fundamentals of dopamine transport and prompt a reevaluation of the impact of K+ on other transporters in this pharmacologically important family. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-20 |
AnnouncementXML | Submission_2022-05-20_08:42:07.317.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kasper Rand |
SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; |
ModificationList | No PTMs are included in the dataset |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-03-20 15:28:57 | ID requested | |
⏵ 1 | 2022-05-20 08:42:08 | announced | |
Publication List
Schmidt SG, Malle MG, Nielsen AK, Bohr SS, Pugh CF, Nielsen JC, Poulsen IH, Rand KD, Hatzakis NS, Loland CJ, The dopamine transporter antiports potassium to increase the uptake of dopamine. Nat Commun, 13(1):2446(2022) [pubmed] |
Keyword List
ProteomeXchange project tag: Hydrogen Deuterium Exchange (HDX-MS) |
submitter keyword: MS |
Membrane proteins |
Mass spectroemetry |
Hydrogen-deuterium exchange |
Transporter |
Contact List
Kasper D. Rand |
contact affiliation | Protein Analysis Group, Department of Pharmacy, University of Copenhagen, Copenhagen, Denmark |
contact email | kasper.rand@sund.ku.dk |
lab head | |
Kasper Rand |
contact affiliation | Department of Pharmacy, University of Copenhagen |
contact email | kasper.rand@sund.ku.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD032663
- Label: PRIDE project
- Name: The dopamine transporter antiports potassium to increase the uptake of dopamine