PXD032406

PXD032406 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	USP18-dependent muscle proteome
Description	USP18 is an interferon type 1 (IFN-1) induced gene that negatively regulates the IFN-1 signalling pathway, which plays a role in many immune related pathologies. Dermatomyositis (DM) is characterised by excessive IFN-1 immune response and muscle degeneration. We found USP18 to be expressed in DM myofibers suggesting a role in muscle cell biology. USP18 depletion induced muscle cell differentiation under nutrient-rich conditions independent of IFN-1 signalling. Downregulation of cell cycle gene network and sarcomeric protein networks, and dysregulation of myogenic (co-)transcription regulators were found in USP18-depleted differentiated cells. Sarcomeric integrity of USP18-depleted cells deteriorated during muscle cell maturation. Our results revealed USP18 as a critical regulator at the intersection between proliferation and differentiation as well as during the maturation of muscle cells. We suggest that a USP18 IFN-1-independent pathway may contribute to muscle regeneration in muscle pathologies.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:46:36.252.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cyriel Olie
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-18 09:47:59	ID requested
1	2023-05-10 13:34:24	announced
⏵ 2	2023-11-14 08:46:44	announced	2023-11-14: Updated project metadata.

Publication List

Olie CS, Pinto-Fern, á, ndez A, Damianou A, Vendrell I, Mei H, den Hamer B, van der Wal E, de Greef JC, Raz V, Kessler BM, USP18 is an essential regulator of muscle cell differentiation and maturation. Cell Death Dis, 14(3):231(2023) [pubmed]

Olie CS, Pinto-Fern, á, ndez A, Damianou A, Vendrell I, Mei H, den Hamer B, van der Wal E, de Greef JC, Raz V, Kessler BM, USP18 is an essential regulator of muscle cell differentiation and maturation. Cell Death Dis, 14(3):231(2023) [pubmed]

Keyword List

submitter keyword: Myogenesis, Muscle Cell Differentiation, Dermatomyositis,USP18, Skeletal Muscle

submitter keyword: Myogenesis, Muscle Cell Differentiation, Dermatomyositis,USP18, Skeletal Muscle

Contact List

Benedikt M. Kessler
contact affiliation	TDI Mass Spectrometry Laboratory, Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Oxford, Roosevelt Drive, Oxford OX3 7FZ, UK
contact email	benedikt.kessler@ndm.ox.ac.uk
lab head
Cyriel Olie
contact affiliation	LUMC
contact email	c.s.olie@lumc.nl
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD032406

PRIDE project URI

Repository Record List

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