PXD032403

PXD032403 is an original dataset announced via ProteomeXchange.

Title	Predictive data-driven modeling of C-terminal tyrosine function in the EGFR signaling network
Description	The Epidermal Growth Factor Receptor (EGFR) has been studied extensively due to its critical role in cellular signaling and association with disease. Previous models have elucidated interactions between EGFR and downstream adaptor proteins, or showed phenotypes affected by EGFR. However, the link between EGFR phosphorylation and phenotypic outcome is still poorly understood. Here, we employed a suite of isogenic cells lines expressing site-specific mutations at each of the EGFR C-terminal phosphorylation sites to interrogate their role in signaling network and cell biological response to stimulation. Our results demonstrate the resilience of the EGFR network, which was largely similar even in the context of multiple Y-to-F mutations in the EGFR C-terminal tail, while also revealing nodes in the network that have not previously been linked to EGFR signaling. Our data-driven model highlights signaling network nodes associated with distinct EGF-driven cell responses, including migration, proliferation, and receptor trafficking. Application of this same approach to less studied RTKs should provide a plethora of novel associations that should lead to a much better understanding of these signaling networks.
HostingRepository	PRIDE
AnnounceDate	2023-05-09
AnnouncementXML	Submission_2023-05-09_09:00:20.645.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD032403
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	JacquelineGerritsen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-18 09:15:23	ID requested
⏵ 1	2023-05-09 09:00:21	announced

10.6019/PXD032403;

submitter keyword: EGFR Phosphoproteomics Phosphorylation Signaling Tyrosine

Forest MichaelWhite
contact affiliation	Department of Biological Engineering Massachusetts Institute of Technology Cambridge MA 02139, Koch Institute for Integrative Cancer Research Massachusetts Institute of Technology Cambridge MA 02142 USA, Center for Precision Cancer Medicine Massachusetts Institute of Technnology Cambridge MA 02142 USA
contact email	fwhite@mit.edu
lab head
JacquelineGerritsen
contact affiliation	MIT Graduate student
contact email	jgn@mit.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032403
     1. Label: PRIDE project
     2. Name: Predictive data-driven modeling of C-terminal tyrosine function in the EGFR signaling network