PXD032394

PXD032394 is an original dataset announced via ProteomeXchange.

Title	Comparison of performances of automatic versus manual liquid handling in density-based separation of extracellular vesicles from plasma and urine
Description	Extracellular vesicles (EV) in body fluids are extensively studied as potential biomarkers for numerous diseases. Major impediments of EV-based biomarker discovery include the manual labor, and the specificity and reproducibility of EV sample preparation. To tackle this, we present an automated liquid handling workstation for the density-based separation of EV from human body fluids and compare its performance to manual handling by (in)experienced researchers. To validate automated density-based separation of EVs from human body fluids, including blood plasma and urine, we assess variation, yield and purity by mass spectrometry-based proteomics.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:55:24.954.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pekka Rappu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-18 09:01:21	ID requested
1	2023-05-09 23:38:49	announced
⏵ 2	2023-11-14 08:55:25	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Plasma, urine, extracellular vesicle, automatic liquid handling

Olivier De Wever
contact affiliation	Laboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University, Ghent, Belgium
contact email	Olivier.DeWever@UGent.be
lab head
Pekka Rappu
contact affiliation	University of Turku
contact email	pekka.rappu@utu.fi
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032394
     1. Label: PRIDE project
     2. Name: Comparison of performances of automatic versus manual liquid handling in density-based separation of extracellular vesicles from plasma and urine