PXD032360

PXD032360 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	MS-based proteomic characterization of Toxoplasma gondii GRA28-associated complexes
Description	The obligate intracellular protozoan Toxoplasma gondii exploits the trafficking of mononuclear phagocytes for systemic dissemination. We report that, upon T. gondii infection, macrophages initiate expression of transcription factors normally attributed to DCs, upregulate the expression of Ccr7 with a chemotactic response, and perform systemic migration when adoptively transferred into mice. We identify the parasite effector GRA28, which is secreted into the host cell nuclei, as driving the chemotactic migratory activation of parasitized macrophages. To gain insight about the molecular role played by GRA28 in this phenomenon, we characterized its interactome.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:18:28.089.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD032360
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: Toxoplasma gondii RH; NCBI TaxID: 383379; scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-17 04:22:55	ID requested
1	2022-11-02 03:30:44	announced
⏵ 2	2023-11-14 07:18:29	announced	2023-11-14: Updated project metadata.

Publication List

10.6019/PXD032360;
Ten Hoeve AL, Braun L, Rodriguez ME, Olivera GC, Bougdour A, Belmudes L, Cout, é Y, Saeij JPJ, Hakimi MA, Barragan A, The Toxoplasma effector GRA28 promotes parasite dissemination by inducing dendritic cell-like migratory properties in infected macrophages. Cell Host Microbe, 30(11):1570-1588.e7(2022) [pubmed]

10.6019/PXD032360;

Ten Hoeve AL, Braun L, Rodriguez ME, Olivera GC, Bougdour A, Belmudes L, Cout, é Y, Saeij JPJ, Hakimi MA, Barragan A, The Toxoplasma effector GRA28 promotes parasite dissemination by inducing dendritic cell-like migratory properties in infected macrophages. Cell Host Microbe, 30(11):1570-1588.e7(2022) [pubmed]

Keyword List

submitter keyword: chemotaxis,Apicomplexa, affinity purification, GRA28, intracellular parasitism, nanoLC-MSMS, host-pathogen

submitter keyword: chemotaxis,Apicomplexa, affinity purification, GRA28, intracellular parasitism, nanoLC-MSMS, host-pathogen

Contact List

Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
lab head
Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD032360
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD032360

PRIDE project URI

Repository Record List

[ + ]