PXD032350

PXD032350 is an original dataset announced via ProteomeXchange.

Title	In vivo prenylomic profiling in the brain of a transgenic mouse model of Alzheimer’s disease reveals increased prenylation of a key set of proteins
Description	Dysregulated prenylation has been implicated in the many diseases, including Alzheimer’s disease (AD). Prenylomic analysis, the combination of metabolic incorporation of an isoprenoid analogue (C15AlkOPP) into prenylated proteins with a bottom-up proteomic analysis, has allowed identification of prenylated proteins in various cellular models. Here, transgenic AD mice were treated with C15AlkOPP through intracerebroventricular (ICV) infusion over 13 days. Using prenylomic analysis, 37 prenylated proteins were enriched in the brains of AD mice . Importantly, the prenylated forms of 15 proteins were consistently upregulated in AD mice compared to non-transgenic wild-type controls. These results highlight the power of this in vivo metabolic labeling approach to identify multiple post-translationally modified proteins that may serve as potential therapeutic targets for a disease that has proved refractory to treatment thus far. Moreover, this method should be applicable to many other types of protein modifications, significantly broadening its scope.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:36:33.887.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shelby Auger
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	farnesylated residue; geranylgeranylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-17 04:11:39	ID requested
1	2022-11-03 10:46:21	announced
⏵ 2	2023-11-14 07:36:34	announced	2023-11-14: Updated project metadata.

Jeong A, Auger SA, Maity S, Fredriksen K, Zhong R, Li L, Distefano MD, Prenylomic Profiling in the Brain of a Transgenic Mouse Model of Alzheimer's Disease Reveals Increased Prenylation of a Key Set of Proteins. ACS Chem Biol, 17(10):2863-2876(2022) [pubmed]

submitter keyword: altzhimer's Disease, brain, prenylation, prenylomics,Mice

Mark, D, Distefano
contact affiliation	Chemistry Department, University of Minnesota, United States
contact email	diste001@umn.edu
lab head
Shelby Auger
contact affiliation	University of Minnesota
contact email	auger054@umn.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD032350

PRIDE project URI

• PRIDE
  1. PXD032350
     1. Label: PRIDE project
     2. Name: In vivo prenylomic profiling in the brain of a transgenic mouse model of Alzheimer’s disease reveals increased prenylation of a key set of proteins