PXD032340
PXD032340 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteome-level characterization of bifunctional peroxidase 4-coumarate 3-hydroxylase knockdown in Brachypodium distachyon provides insights into lignin modification-associated pleiotropic effects |
| Description | A bifunctional peroxidase enzyme, 4-coumarate 3-hydroxylase (C3H/APX), provides a parallel route to the shikimate shunt pathway for the conversion of 4-coumarate to caffeate in the early steps of lignin biosynthesis. Knockdown of C3H/APX (c3h/apx) expression has been shown to reduce the lignin content in Brachypodium distachyon. However, like many other lignin-modified plants, c3h/apx plant shows unpredictable pleiotropic phenotypes, including stunted growth, delayed senescence, and reduced seed yield. A system-wide level understanding of altered biological processes in lignin-modified plants can help pinpoint the lignin-modification associated growth defects to benefit future studies aiming to negate the yield penalty. Here, a global proteomics measurement of the stem tissue of the model grass Brachypodium distachyon was used to investigate the underlying mechanism of c3h/apx-associated lignin modification and negative growth phenotype. Our findings demonstrate that C3H/APX knockdown in Brachypodium stems substantially alters the abundance of enzymes implicated in the phenylpropanoid biosynthetic pathway and disrupt cellular redox homeostasis. Moreover, it elicits plant defense response associated with intracellular kinases and phytohormone-based signaling to facilitate growth-defense trade-offs. A deeper understanding along with potential targets to mitigate the pleiotropic phenotypes identified in this study could aid to increase the economic feasibility of lignocellulosic biofuel production. |
| HostingRepository | MassIVE |
| AnnounceDate | 2022-09-28 |
| AnnouncementXML | Submission_2022-09-28_06:33:36.939.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | CBI_lignin_c3h |
| SpeciesList | scientific name: Brachypodium distachyon; common name: stiff brome; NCBI TaxID: 15368; |
| ModificationList | Carbamidomethyl; Oxidation |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-03-16 11:47:31 | ID requested | |
| ⏵ 1 | 2022-09-28 06:33:37 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: LC-MS/MS, comparative proteomics, Brachypodium distachyon, lignin biosynthesis, cytosolic ascorbate peroxidase |
Contact List
| Paul E. Abraham | |
|---|---|
| contact affiliation | Oak Ridge National Laboratory |
| contact email | abrahampe@ornl.gov |
| lab head | |
| Jaime Barros | |
| contact affiliation | University of North Texas |
| contact email | jaime.Barros-Rios@unt.edu |
| lab head | |
| CBI_lignin_c3h | |
| contact affiliation | ORNL |
| contact email | abrahampe@ornl.gov |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000089074/ |
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