PXD032334 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic characterization of SEC-fractionated sEV subpopulations |
Description | The use of cardiac progenitor cell (CPC)-derived small extracellular vesicles (sEVs) has shown potential to stimulate cardiac repair. sEVs are released by cells and play a role in intercellular communication through transfer of their bioactive content. Increasing evidence indicates that sEVs present a heterogeneous population of vesicles. In the context of cardiac regeneration, studying sEV heterogeneity could provide new insights into mechanisms underlying sEV-mediated cardiac repair properties and help to improve the therapeutic application of CPC-derived sEVs. Here we used size-exclusion chromatography to isolate and fractionate EV subpopulations dervied from CPCs. We then applied a bottom-up proteomic approach to characterize the contents of individual EV subpopulations. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:25:10.203.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Julia Bauzá Martinez |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-03-16 09:18:12 | ID requested | |
1 | 2024-01-26 06:09:01 | announced | |
⏵ 2 | 2024-10-22 06:25:19 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1002/jev2.12396; |
van de Wakker SI, Bauz, á, -Martinez J, R, í, os Arceo C, Manjikian H, Snijders Blok CJB, Roefs MT, Willms E, Maas RGC, Pronker MF, de Jong OG, Wu W, G, ö, rgens A, El Andaloussi S, Sluijter JPG, Vader P, Size matters: Functional differences of small extracellular vesicle subpopulations in cardiac repair responses. J Extracell Vesicles, 13(1):e12396(2024) [pubmed] |
Keyword List
submitter keyword: size exclusion chromatography, bottom-up proteomics,Extracellular vesicles, CPC |
Contact List
Wei Wu |
contact affiliation | Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands; Netherlands Proteomics Centre, Padualaan 8, 3584 CH Utrecht, The Netherlands; and Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore. |
contact email | wu_wei@immunol.a-star.edu.sg |
lab head | |
Julia Bauzá Martinez |
contact affiliation | Utrecht University |
contact email | j.bauzamartinez@uu.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD032334
- Label: PRIDE project
- Name: Proteomic characterization of SEC-fractionated sEV subpopulations