PXD032328

PXD032328 is an original dataset announced via ProteomeXchange.

Title	TMEM260 is a protein-specific O-mannosyltransferase
Description	Mutations in the TMEM260 gene cause structural heart defects and renal anomalies syndrome (SHDRA), but the function of the encoded protein remains unknown. We report that TMEM260 is an ER-located protein O-mannosyltransferase that selectively glycosylates defined extracellular immunoglobulin, plexin, transcription factor (IPT) domains of the hepatocyte growth factor receptor (cMET), macrophage-stimulating protein receptor (RON), and plexin receptors. We demonstrate that disease-causing TMEM260 mutations impair O-mannosylation of IPT domains and that TMEM260 knock out in cells results in receptor maturation defects and abnormal growth of 3D cell models. Thus, our study identifies a new, receptor-specific O-mannosylation pathway that serves critical functions during e.g. epithelial morphogenesis.
HostingRepository	PRIDE
AnnounceDate	2023-05-05
AnnouncementXML	Submission_2023-05-05_06:07:21.137.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	AdnanHalim
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; hexosylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-16 09:10:53	ID requested
⏵ 1	2023-05-05 06:07:21	announced

Dataset with its publication pending

submitter keyword: O-mannosylation, glycoproteomics, glycopeptide,TMEM260

AdnanHalim
contact affiliation	Copenhagen Center for Glycomics (CCG), Department of Cellular and Molecular Medicine, University of Copenhagen, Denmark
contact email	halim@sund.ku.dk
lab head
AdnanHalim
contact affiliation	University of Copenhagen
contact email	halim@sund.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD032328

PRIDE project URI

• PRIDE
  1. PXD032328
     1. Label: PRIDE project
     2. Name: TMEM260 is a protein-specific O-mannosyltransferase