PXD032321

PXD032321 is an original dataset announced via ProteomeXchange.

Title	Oxygen level regulates protein N-terminal translation elongation through deoxyhypusine hydroxylation
Description	Hypusine is a post-translational modification on eukaryotic translation initiation factor 5A (eIF5A). The last step in the biosynthesis of hypusine, deoxyhypusine hydroxylation, is an oxygen dependent reaction. Here we show that deletion of deoxyhypusine hydroxylase, Lia1, compromises yeast respiration through translation downregulation of proteins in respiration pathway. The translation suppression, due to the lack of deoxyhypusine hydroxylation, mainly affects the translation of the N-termini of the proteins, which is independent of the presence of proline residues, but likely dependent on the interaction between the N-terminal nascent peptide and the ribosomal peptidyl exiting tunnel. Proteomics and biochemical studies revealed that Lia1 deletion decreased the N-terminal translation of proteins involved in mitochondrial respiration, oxidative stress response, and heat shock response. Our work uncovers previously unknown functions of the hypusine modification by considering the substrate requirement of the post-translation modification, highlights the unique challenges of translating the N-termini of proteins and demonstrates a novel oxygen sensing mechanism in eukaryotic cells.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-12_00:24:34.507.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sheng Zhang
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	hypusine
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-16 04:13:19	ID requested
⏵ 1	2022-08-12 00:24:35	announced

Zhang Y, Su D, Zhu J, Wang M, Zhang Y, Fu Q, Zhang S, Lin H, Oxygen level regulates N-terminal translation elongation of selected proteins through deoxyhypusine hydroxylation. Cell Rep, 39(8):110855(2022) [pubmed]

submitter keyword: Oxygen, hypusine, deoxyhupusine, SILAC

Sheng Zhang
contact affiliation	Cornell University
contact email	sz14@cornell.edu
lab head
Sheng Zhang
contact affiliation	Cornell University
contact email	sz14@cornell.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032321
     1. Label: PRIDE project
     2. Name: Oxygen level regulates protein N-terminal translation elongation through deoxyhypusine hydroxylation