PXD032319

PXD032319 is an original dataset announced via ProteomeXchange.

Title	Toxoplasma gondii PRU ROP1-HA Co-Immunoprecipitation
Description	Toxoplasma gondii is an intracellular parasite that can infect many different host species and is a cause of significant human morbidity worldwide. T. gondii secretes a diverse array of effector proteins into the host cell which are critical for infection; however, the vast majority of these secreted proteins are uncharacterised. Here, we carried out a pooled CRISPR knockout screen in the T. gondii Prugniaud strain in vivo to identify secreted proteins which contribute to parasite immune evasion in the host. We identify 22 putative virulence factors and demonstrate that ROP1, the first-identified rhoptry protein of T. gondii, has a previously unrecognised role in parasite resistance to interferon gamma-mediated innate immune restriction. This function is conserved in the highly virulent RH strain of T. gondii and contributes to parasite growth in both murine and human macrophages. While ROP1 affects the morphology of rhoptries, from where the protein is secreted, it does not affect rhoptry secretion. Instead, ROP1 interacts with the host cell protein C1QBP, which appears to facilitate parasite immune evasion. In summary, we identify 22 secreted proteins which contribute to parasite growth in vivo and show that ROP1 is an important and previously overlooked effector in counteracting both murine and human innate immunity.
HostingRepository	PRIDE
AnnounceDate	2022-10-20
AnnouncementXML	Submission_2022-10-20_03:19:00.064.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	SimonButterworth
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Toxoplasma gondii PRU; NCBI TaxID: 1080348;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-16 04:12:40	ID requested
⏵ 1	2022-10-20 03:19:00	announced

Dataset with its publication pending

submitter keyword: Toxoplasma gondii

MoritzTreeck
contact affiliation	Signalling in Apicomplexan Parasites Laboratory, The Francis Crick Institute, London NW1 1AT, UK
contact email	moritz.treeck@crick.ac.uk
lab head
SimonButterworth
contact affiliation	The Francis Crick Institute
contact email	simon.butterworth@crick.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032319
     1. Label: PRIDE project
     2. Name: Toxoplasma gondii PRU ROP1-HA Co-Immunoprecipitation