PXD032313

PXD032313 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	A comparison of blood plasma exosome enrichment strategies for proteomic analysis.
Description	Proteomic analysis of exosomes (EXs) poses a significant challenge. A ‘gold-standard’ method for plasma EX enrichment for downstream proteomic analysis is yet to be established. Methods were evaluated for their capacity to successfully isolate and enrich EXs from plasma, minimise the presence of highly abundant plasma proteins, and result in the optimum representation of exosomal (EXal) proteins by liquid chromatography tandem mass spectrometry. Plasma from four cattle (Bos taurus) of similar physical attributes and genetics were used. Three methods of EX enrichment were utilised: ultracentrifugation (UC), size-exclusion chromatography (SEC), and ultrafiltration (UF). These methods were combined to create four groups for methodological evaluation: UC+SEC, UC+SEC+UF, SEC+UC and SEC+UF. The UC+SEC method yielded the highest number of protein IDs.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-12_00:03:21.124.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Natalie Turner
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-16 03:58:36	ID requested
⏵ 1	2022-08-12 00:03:21	announced

Publication List

Turner NP, Abeysinghe P, Kwan Cheung KA, Vaswani K, Logan J, Sadowski P, Mitchell MD, A Comparison of Blood Plasma Small Extracellular Vesicle Enrichment Strategies for Proteomic Analysis. Proteomes, 10(2):(2022) [pubmed]

Turner NP, Abeysinghe P, Kwan Cheung KA, Vaswani K, Logan J, Sadowski P, Mitchell MD, A Comparison of Blood Plasma Small Extracellular Vesicle Enrichment Strategies for Proteomic Analysis. Proteomes, 10(2):(2022) [pubmed]

Keyword List

submitter keyword: Exosome
isolation
enrichment
ultracentrifugation
size-exclusion chromatography
ultrafiltration
mass-spectrometry
proteomics
extracellular vesicles

submitter keyword: Exosome

isolation

enrichment

ultracentrifugation

size-exclusion chromatography

ultrafiltration

mass-spectrometry

proteomics

extracellular vesicles

Contact List

Pawel Sadowski
contact affiliation	CARF Proteomics and Small Molecule Mass Spectrometry, Queensland University of Technology
contact email	pawel.sadowski@qut.edu.au
lab head
Natalie Turner
contact affiliation	Queensland University of Technology
contact email	natalie.turner@hdr.qut.edu.au
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD032313
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD032313

PRIDE project URI

Repository Record List

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