PXD032305

PXD032305 is an original dataset announced via ProteomeXchange.

Title	High-throughput and high-sensitivity biomarker monitoring in body fluid by fast LC SureQuant™ IS targeted quantitation
Description	Targeted proteomics methods have been greatly improved and refined over the last decade and are becoming increasingly the method of choice in protein and peptide quantitative assays. Despite the tremendous progress in targeted proteomic assays, they still suffer from inadequate sensitivity for lower abundant proteins and throughput, especially in complex biological samples. These attributes are essential for establishing targeted proteomics methods at the forefront of clinical use. Here, we report an assay utilizing the SureQuantTM internal standard triggered targeted method on a contemporary mass spectrometer coupled with an EvoSep One liquid chromatography platform, which displays markedly enhanced sensitivity and a high throughput of 100 samples per day. We demonstrate the robustness of this method by quantifying proteins ranging six orders of magnitude in human wound fluid exudates, a biological fluid that exhibits sample complexity and composition similar to plasma. Among the targets quantified were low abundant proteins such at TNFA and IL1B, highlighting the value of this method in the quantification of trace amounts of invaluable biomarkers that were until recently outside of the sphere of quantification with targeted proteomics methods. Taken together, this method provides an excellent addition to the toolkit of targeted proteomic assays and has the potential to be a centerpiece in biomarker quantification studies.
HostingRepository	PanoramaPublic
AnnounceDate	2025-10-07
AnnouncementXML	Submission_2025-10-07_09:12:46.828.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Konstantinos Kalogeropoulos
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-15 11:11:25	ID requested
⏵ 1	2025-10-07 09:12:47	announced

Kalogeropoulos K, Savickas S, Haack AM, Larsen CA, Mikosi, ń, ski J, Schoof EM, Smola H, Bundgaard L, Auf dem Keller U, High-Throughput and High-Sensitivity Biomarker Monitoring in Body Fluid by Fast LC SureQuant IS-Targeted Quantitation. Mol Cell Proteomics, 23(12):100868(2024) [pubmed]

submitter keyword: targeted proteomics, biofluids, biomarker quantification, IS-PRM, SureQuant, high throughput

Konstantinos Kalogeropoulos
contact affiliation	Technical University of Denmark
contact email	uadk@dtu.dk
lab head
Konstantinos Kalogeropoulos
contact affiliation	Technical University of Denmark
contact email	uadk@dtu.dk
dataset submitter

Panorama Public dataset URI