PXD032302

PXD032302 is an original dataset announced via ProteomeXchange.

Title	FIP-fve stimulates cell proliferation and enhances IL-2 release by activating MAP2K3/p38α（MAPK14）signaling pathway in Jurkat E6-1 cells
Description	FIP-fve, a fungal fruiting body protein from Flammulina velutipes, has potential immunomodulatory properties. Here, we investigated the immunomodulation mechanism of FIP-fve in Jurkat E6-1 cells by conducting cell viability assay and IL-2 release assay. Kinase inhibitors experiment and proteomics analysis were also involved in the mechanism study. It was found that FIP-fve stimulated cell proliferation and enhanced IL-2 secretion in a dose-dependent manner in Jurkat E6-1 cells. Unbiased high-throughput proteomics analysis showed that 4 T cell immune activation markers including ZAP-70, CD69, CD82 and KIF23 were upregulated in response to FIP-fve treatment. Further pathway analysis indicated that MAP2K3/p38 pathway related proteins including MAP2K, p38, ELK, AATF, FOS, and JUN-B were unregulated. In addition, losmapimod (p38 inhibitor) and gossypetin (MAP2K3 inhibitor) inhibited FIP-fve enhanced cell proliferation and IL-2 release in Jurkat E6-1 cells. Our results demonstrate that FIP-fve stimulates cell proliferation and enhances IL-2 secretion through MAP2K3/ p38α activation.
HostingRepository	PRIDE
AnnounceDate	2022-06-09
AnnouncementXML	Submission_2022-06-09_09:13:49.782.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	kefei GU
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-15 03:36:14	ID requested
⏵ 1	2022-06-09 09:13:50	announced

Gu K, Wang T, Peng L, Zhao Y, (MAPK14) Signaling Pathway in Jurkat E6-1 Cells. Front Nutr, 9():881924(2022) [pubmed]

submitter keyword: FIP-fve, immunomodulation mechanism, proteomics analysis, MAP2K3/p38α, IL-2

Kefei GU
contact affiliation	Institute for Agri-food Standards & Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai, China
contact email	xyz694@163.com
lab head
kefei GU
contact affiliation	Institute for Agri-food Standards & Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai, China
contact email	80540253@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032302
     1. Label: PRIDE project
     2. Name: FIP-fve stimulates cell proliferation and enhances IL-2 release by activating MAP2K3/p38α（MAPK14）signaling pathway in Jurkat E6-1 cells