PXD032297

PXD032297 is an original dataset announced via ProteomeXchange.

Title	Pattern Analysis of Organellar Maps for Interpretation of Pro-teomic Data
Description	The human proton-coupled folate transporter (PCFT) was stably expressed as a fu-sion protein with a BioID2-HA in the C-terminal position (PCFT-BioID2). The uncom-plxed biotin transferase was used as a control to identify proteins that are biotinylated randomly due to sheer abundance and/or an unnatural affinity to the BioID2 ligase. HeLa PCFT-BioID2 cells expressed the PCFT fusion protein which localized to the plasma mem-brane and exhibited robust transport typical of PCFT. HeLa PCFT-BioID2 and BioID2 cells were cultured in complete DMEM (biotin-free), with and without biotin (50 μM; 16 h) then biotinylated proteins were selected on streptavidin beads.
HostingRepository	PRIDE
AnnounceDate	2022-06-09
AnnouncementXML	Submission_2022-06-09_09:48:22.010.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD032297
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jordan Burton
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-15 03:33:54	ID requested
⏵ 1	2022-06-09 09:48:22	announced

Burton JB, Carruthers NJ, Hou Z, Matherly LH, Stemmer PM, Pattern Analysis of Organellar Maps for Interpretation of Proteomic Data. Proteomes, 10(2):(2022) [pubmed]

submitter keyword: Proteomics

Pattern Analysis

Bioinformatics

Proximity Labeling

Paul M. Stemmer
contact affiliation	Wayne State University, Detroit, MI, USA
contact email	pmstemmer@wayne.edu
lab head
Jordan Burton
contact affiliation	Department of Chemistry Institute of Environmental Health Sciences Wayne State University
contact email	jordan.burton@wayne.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD032297

PRIDE project URI

• PRIDE
  1. PXD032297
     1. Label: PRIDE project
     2. Name: Pattern Analysis of Organellar Maps for Interpretation of Pro-teomic Data