PXD032295

PXD032295 is an original dataset announced via ProteomeXchange.

Title	Experimental maceration of bovine bones and its effects on the bone proteome
Description	This study is aimed at understanding how bone soft maceration procedures (e.g., soaking the bones in warm water with the aid of detergents) used to process bones for long term storage (e.g., in human osteological collections) affect the bone proteome. Three different maceration protocols have been tested in the study, in order to test different lenghts for the soaking in water, different temperatures and different detergents. Bovine tibiae (n=6, 3 left and 3 right from 3 animals) have been used in the experiment - in particular, the left ones have been used as non-macerated controls, and the right ones have been macerated with three protocols (one per protocol). Sampling was conducted on both fresh and macerated remains and used to conduct shotgun proteomics analyses.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:02:29.867.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD032295
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Noemi Procopio
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-15 03:33:15	ID requested
1	2023-03-08 09:27:09	announced
⏵ 2	2023-11-14 08:02:30	announced	2023-11-14: Updated project metadata.

10.6019/PXD032295;

Gent L, Schwalbe EC, Procopio N, The impact of maceration on the 'Osteo-ome'

a pilot investigation. J Proteomics, 271():104754(2023) [pubmed]

submitter keyword: maceration,Bone, Shotgun proteomics

Noemi Procopio
contact affiliation	Forensic Science Research Group, Faculty of Health and Life Sciences, Applied Sciences, Northumbria University, NE1 8ST, Newcastle Upon Tyne, UK
contact email	noemi.procopio@northumbria.ac.uk
lab head
Noemi Procopio
contact affiliation	University of Central Lancashire
contact email	noemi.procopio@northumbria.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD032295

PRIDE project URI

• PRIDE
  1. PXD032295
     1. Label: PRIDE project
     2. Name: Experimental maceration of bovine bones and its effects on the bone proteome