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PXD032265

PXD032265 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBroad-range metalloprotease profiling in plants uncovers immunity provided by defence-related metalloenzyme
DescriptionPlants encode for >100 metalloproteases representing >19 different protein families. Tools to study this large and diverse class of proteases have not yet been introduced into plant science. Here, we used hydroxamate-based photoaffinity probes to explore plant proteomes for metalloproteases. We detected labeling of 23 metalloproteases in leaf extracts of the model plant Arabidopsis thaliana that belong to eight unrelated metalloprotease families and localize to different subcellular compartments. We identified several chloroplast localized FtsH proteases; vacuolar aspartyl aminopeptidase DAP1; peroxisomal metalloprotease PMX16; and many cytosolic metalloproteases. We also identified non-proteolytic metallohydrolases involved the release of auxin and in the urea cycle. Studies on tobacco plant Nicotiana benthamiana infected with the bacterial plant pathogen Pseudomonas syringae uncovered PRp27, a secreted protein with anticipated metalloprotease activity. PRp27 depletion increases susceptibility, whilst its overexpression increases resistance, dependent on the presence of active site residues. Collectively, these studies uncover the strength of hydroxamate-based probes to study metalloenzymes in plants
HostingRepositoryPRIDE
AnnounceDate2022-08-12
AnnouncementXMLSubmission_2022-08-12_00:03:41.451.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKyoko Morimto
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; scientific name: Nicotiana benthamiana; NCBI TaxID: 4100;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-03-14 09:41:41ID requested
12022-08-12 00:03:42announced
Publication List
Morimoto K, Krahn D, Kaschani F, Hopkinson-Woolley D, Gee A, Buscaill P, Mohammed S, Sieber SA, Cravatt BF, Schofield CJ, van der Hoorn RAL, Broad-range metalloprotease profiling in plants uncovers immunity provided by defence-related metalloenzyme. New Phytol, 235(3):1287-1301(2022) [pubmed]
Keyword List
submitter keyword: Arabidopsis, metalloproteases, photoaffinity labelling, PRp27, immunity
Contact List
Renier A. L. van der Hoorn1
contact affiliationThe Plant Chemetics Laboratory, Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, United Kingdom
contact emailrenier.vanderhoorn@plants.ox.ac.uk
lab head
Kyoko Morimto
contact affiliationKyomei
contact emailkyoko@kyomei.co.uk
dataset submitter
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