PXD032265 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Broad-range metalloprotease profiling in plants uncovers immunity provided by defence-related metalloenzyme |
Description | Plants encode for >100 metalloproteases representing >19 different protein families. Tools to study this large and diverse class of proteases have not yet been introduced into plant science. Here, we used hydroxamate-based photoaffinity probes to explore plant proteomes for metalloproteases. We detected labeling of 23 metalloproteases in leaf extracts of the model plant Arabidopsis thaliana that belong to eight unrelated metalloprotease families and localize to different subcellular compartments. We identified several chloroplast localized FtsH proteases; vacuolar aspartyl aminopeptidase DAP1; peroxisomal metalloprotease PMX16; and many cytosolic metalloproteases. We also identified non-proteolytic metallohydrolases involved the release of auxin and in the urea cycle. Studies on tobacco plant Nicotiana benthamiana infected with the bacterial plant pathogen Pseudomonas syringae uncovered PRp27, a secreted protein with anticipated metalloprotease activity. PRp27 depletion increases susceptibility, whilst its overexpression increases resistance, dependent on the presence of active site residues. Collectively, these studies uncover the strength of hydroxamate-based probes to study metalloenzymes in plants |
HostingRepository | PRIDE |
AnnounceDate | 2022-08-12 |
AnnouncementXML | Submission_2022-08-12_00:03:41.451.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kyoko Morimto |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; scientific name: Nicotiana benthamiana; NCBI TaxID: 4100; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-03-14 09:41:41 | ID requested | |
⏵ 1 | 2022-08-12 00:03:42 | announced | |
Publication List
Morimoto K, Krahn D, Kaschani F, Hopkinson-Woolley D, Gee A, Buscaill P, Mohammed S, Sieber SA, Cravatt BF, Schofield CJ, van der Hoorn RAL, Broad-range metalloprotease profiling in plants uncovers immunity provided by defence-related metalloenzyme. New Phytol, 235(3):1287-1301(2022) [pubmed] |
Keyword List
submitter keyword: Arabidopsis, metalloproteases, photoaffinity labelling, PRp27, immunity |
Contact List
Renier A. L. van der Hoorn1 |
contact affiliation | The Plant Chemetics Laboratory, Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, United Kingdom |
contact email | renier.vanderhoorn@plants.ox.ac.uk |
lab head | |
Kyoko Morimto |
contact affiliation | Kyomei |
contact email | kyoko@kyomei.co.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD032265
- Label: PRIDE project
- Name: Broad-range metalloprotease profiling in plants uncovers immunity provided by defence-related metalloenzyme