PXD032260

PXD032260 is an original dataset announced via ProteomeXchange.

Title	C. elegans phosphoproteomics - DAF15 knockdown (The TORC1 phosphoproteome in C. elegans reveals roles in transcription and autophagy)
Description	The protein kinase complex Target of Rapamycin complex 1 (TORC1) is a critical mediator of nutrient sensing that has been widely studied in cultured cells and yeast, yet our understanding of the regulatory activities of TORC1 in the context of a whole, multi-cellular organism is still very limited. Using C. elegans, we analyzed the DAF-15/Raptor-dependent phosphoproteome by quantitative mass spectrometry and characterized direct kinase targets by in vitro kinase assays. Here we show new targets of TORC1 that indicate previously unknown regulation of transcription and autophagy. Our results further show that DAF-15/Raptor is differentially expressed during postembryonic development, suggesting a dynamic role for TORC1 signaling throughout development. This study provides a comprehensive view of the TORC1 phosphoproteome and reveals more than 100 DAF-15/Raptor-dependent phosphosites, reflecting the complex function of TORC1 in a whole, multi-cellular organism.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:55:21.720.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zach Poss
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	phosphorylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-14 08:55:57	ID requested
1	2022-04-22 09:30:09	announced
2	2022-04-25 01:55:16	announced	2022-04-25: Updated publication reference for DOI(s): doi.org/10.1016/j.isci.2022.104186.
⏵ 3	2023-11-14 08:55:22	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: autophagy,C. elegans, phosphoproteome, mTORC1, DAF-15, raptor, mTOR

Min Han
contact affiliation	Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309-0347 USA
contact email	mhan@colorado.edu
lab head
Zach Poss
contact affiliation	University of Colorado
contact email	possz@colorado.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD032260

PRIDE project URI

• PRIDE
  1. PXD032260
     1. Label: PRIDE project
     2. Name: C. elegans phosphoproteomics - DAF15 knockdown (The TORC1 phosphoproteome in C. elegans reveals roles in transcription and autophagy)